All ActiGraphs have a non-volatale (NVRAM) on flash based memory. Collected data are stored at all times, execpt for unexpected battery failure. 

The graphs below show for every device what the total collecting time (in days) is:


storage capacity GT1M

storage capacity GT3X (4MB)

storage capacity GT3X+ (16MB)

 

storage capacity ActiSleep Monitor (ASM) (4MB)

 

Storage capacity ActiTrainer (2-assig, 1MB) 


Storage capacity ActiTrainer (3-assig, 4MB)

http://support.theactigraph.com/faq/actilife-quote-remover-tool

Possible error message:
During the export from a file in CVS format, the output (data in the CVS-file) can hale double quotation marks

Problem:
ActiLife send all CSV-files inaccurately with double quotation marks 

Solution:
Download the above mentioned programm and select the file(s) you want to repair. This problem is solveld after version 5.7.1

What's different?

ActiLife will produce the same cut point categorization result regardless of epoch length beginning with version 6.8.  
With versions 6.7.1 and older, ActiLife categorized epoch values into cut point "buckets" by scaling the count level up to its 60s equivalent.  The example shown below summarizes the difference in cut point results for a 10s epoch file and its 60s equivalent over a period of 5 minutes.  Note the 10s breakdown of cut point results for the smaller epoch file.

OLD WAY (ActiLife 6.7.1 and older [before August 2013])

























With ActiLife 6.8 and newer, the results will be identical, regardless of the file's epoch length.  

NEW WAY (ActiLife 6.8.0 and newer [After August 2013])



























The figure below shows the differences in how ActiLife will calculate results 
 

Why did we change?

After speaking with several industry leaders, we determined that the cut points themselves were not designed to be compared to scaled, sub-60s epoch count levels.  Documentation outlined in the help article "What's the difference among the Cut Points available in ActiLife?" further validates this decision. All of the cut points supported in ActiLife were designed to be used only with 60s files.

What is the impact on my data?
If you have processed cut points in the past using sub-60s epoch files, your results may not be comparable.  Those files can be reprocessed in the new version of ActiLife quickly since we always ensure backward compatibility.  Your original epoch and raw data is never changed by ActiLife so you can always reproduce results at anytime.

We do our best to avoid changing algorithm results but we also want to inform our users if we're forced to do so.  We appreciate your understanding; please feel free to contact us with any questions.

1. On your desktop, right click and select Properties
2. A box will appear called Display Properties. Click on the Settingstab.

3. Click on Advanced



4. Change the DPI setting to Normal Size (96 DPI). If you have the value set to anything else, text and images on in ActiLife will look jumbled on the screen.
Click OK and your computer will ask you to reboot your computer. Click Yes.


To change the setting on Windows Vista\7:
1. On your desktop, right click and select "Personalize"
2. In the new window, towards to bottom left corner, click on the Display link.
3. You will now get a new screen
4. Select Smaller - 100% (default) and click Apply.
5. Click on Log Off button to apply the DPI changes. Log back on to see the changes.

There are two flavours from the ActiLife software, the full and the lite. A full version means that you can do everything with the software that's possible like initialization, import patient information, import data from device to software and analyzing data. The lite version looks exactly the same, but the big difference is that with this version it's only possible to export data and initialize the device again. This saves time and is expecially helpfull in big research projects with more then one researcher involved. Analyzing is not possible with the lite version, the full version has to be used in that case!

As a measure of security and controlling what external resources can be accessed from an Organizations network, some IT Departments may have implemented Proxy Servers. These Servers direct all internet/network traffic through a specific computer which determines, based on the configuration set up by the Administrators, whether that website is accessible to a machine located on that network. This is done to prevent users from accessing websites that could introduce security concerns like viruses or malware, or that are simply deemed inappropriate for use on that network (i.e. Facebook, Netflix, Youtube, game-related sites, etc).

In order to check for program updates, to use the Data Vault, and to verify subscription status (for ActiLife 6.x users), ActiLife does require access via Port 80 (normal web traffic) and Port 443 (secure web traffic). In some instances, these Proxy Servers may block or otherwise obstruct this communication. If you encounter issues activating, opening, or using the features within ActiLife, please first verify with your IT department whether or not they have implemented a Proxy Server and if so, what the address of the Proxy server is and what port all traffic should be redirected to use.

Beginning with ActiLife 6.3, we have added basic Proxy Support. In order to properly configure this, you will need to know what the Proxy server's IP address is, the Port number, and any username or password that may be required. The default settings (i.e. Auto-Detect) might be all that is required but depending on how things are set up, you may need to manually enter this information. You can get to these settings by opening ActiLife and going to Edit, then Options, and selecting Proxy on the left side of the screen. Complete the box and click OK. (Please refer to the following image:)

The ActiSleep software and drivers requires that COM ports 1 thru 16 are available during use. You can checkto see if the device connected to your computer is using the correct COM port and even change it if it isn't.

Please note that the ActiSleep software cannot operate while other ActiGraph software is installed. For example,ActiLife and ActiSleep software clients cannot be installed together on the same computer because the devicedrivers they come with cannot reside and operate simultaniously.

To check and change the COM setting, followthese steps:

1. Open the Control Panel in Windows and double click on "System" Windows XP: Click on the"Hardware tab" and then click on "Device Manager" Windows Vista/7: Click on "Device Manager" in the top leftIn the new window, find "Ports (COM & LPT)" and expand the item but double clicking on it. If you have adevice attached, check to see if it is this list.
2. If it is, check the COM number in the name. If you do not see it inthis list, but have a "? Other Devices" in the list, then you may not have installed the drivers correctly.

To changethe COM number, double click on the device in the list. Click the "Port Settings" tab and then click the"Advanced" button. You will see the "COM Port Number" drop down box in the bottom left. Select a value thatis between 1 and 16. If all of those state "In Use", then select a value between 4 and 8 (even if it states In Use).Click Ok, then OK again and try starting the ActiSleep software.

As of ActiLife version 6.1.x, there are nine sets of Cut Points available to users.  These cut points were derived as part of past published research aimed at quantifying activity levels using ActiGraph products.  Definitions of the cut point levels and references to the corresponding published research are given below.  Note that the published research is only referenced by its abstract; full publications are available for purchase from the corresponding journal.  Note that all cut point sets are scaled to 60s epochs.  Even if the cut point set was originally defined for sub-60s epoch files, the cut points were scaled in ActiLife in CPM; Counts Per Minute.

For an explanation of what a "count" is, please see this article.

https://help.theactigraph.com/entries/20723176-What-are-counts-

Freedson Adult 1998:

• Reference Research
  • Freedson, PS; Melanson, E; Sirard, J; Calibration of the Computer Science and Applications, Inc. Accelerometer (1997, Medicine & Science in Sports Exercise)
  • Link
• Special Notes
  • Though these cut points were derived with legacy versions of our ActiGraph hardware, they still apply today because of backward compatibility of ActiGraph products.  The cut points were derived based on the MET thresholds described in the reference research.  The Lifestyle cut point was added after a verbal discussion with the author regarding the need for an intermediary cut point level.  Contact the author for further explanation.

Freedson Adult VM3 2011

• Reference Research
  • Sasaki, JE; John, D; Freedson, PS.  Validation and Comparison of ActiGraph Activity Monitors
• Special Notes
  • Fifty participants wore the GT3X and the GT1M on the non-dominant hip and exercised at 4 treadmill speeds (4.8, 6.4, 9.7, and 12 km h?1). Vertical (VT) and antero-posterior (AP) activity counts (counts min?1) as well as the vector magnitudes of the two axes (VM2) from both monitors were tested for significant differences using two-way ANOVA’s. Bland–Altman plots were used to assess agreement between activity counts from the GT3X and GT1M. Linear regression analysis between VM3 counts min?1 and oxygen consumption data was conducted to develop VM3 cut-points for moderate, hard and very hard PA.

Freedson Children 2005

• Reference Research
  • Freedson PS, Pober D, Janz KF. Calibration of accelerometer output for children. Med Sci Sports Exerc. 2005;37(11 suppl): S523–30
• Special Notes
  • Studies include walking, running and free-living activities in the testing protocol.
  • These cut points are based off of the MET formula METs = 2.757 + (0.0015*CPM) - (0.08957*age) - (0.000038*CPM*age) with assumed MET thresholds of 3, 6, and 9 METs (which produce cut point boundaries of 500, 4000, and 7600 CPM respecively).  Trost, in Comparison of Accelerometer Cut Points for Predicting Activity Intensity in Youth (Trost, et., al., Medicine & Science in Sports & Exercise - link) assumed the following MET cut points: Sedentary: 0 - 1.78 METs; Light - 1.79 METs - 3.99 METs; Moderate: 4.00 METs - 5.99 METs; Vigorous: >= 6.00 METs.  These thresholds resulted in the following cut points: Sedentary: 0-99 CPM; Light: 100 - 2219 CPM; Moderate: 2220 - 4135 CPM; Vigorous: 4136 - ∞ CPM.  For the sake of the original author's intention, these cut points were not used.  Users can create their own custom cut point set in ActiLife if they choose to use these. 

Puyau Children 2002
 

• Reference Research
  • Puyau MR, Adolph AL, Vohra FA, Butte NF. Validation and calibration of physical activity monitors in children. Obes Res. 2002;10:150–7.
• Special Notes
  • Devices were worn on the hip or lower leg, were validated and calibrated against 6-hour EE measurements by room respiration calorimetry, activity by microwave detector, and heart rate by telemetry in 26 children. 6 to 16 years old. During the 6 hours, the children performed structured activities, including resting metabolic rate (RMR), Nintendo, arts and crafts, aerobic warm-up, Tae Bo, treadmill walking and running, and games. Activity energy expenditure (AFE) computed as EE — RMR was regressed against counts to derive threshold counts.

Teuth Childrens only 2004

• Reference Research
  • Treuth MS, Schmitz K, Catellier DJ, et al. Defining accelerometer thresholds for activity intensities in adolescent girls. Med Sci Sports Exerc. 2004;36(7):1259–66.
• Special Notes
  • Seventy-four healthy 8th grade girls, age 13-14 yr, were recruited from urban areas of Baltimore, MD, Minneapolis/St. Paul, MN, and Columbia, SC, to participate in the study. Accelerometer and oxygen consumption [latin capital V with dot above]02) data for 10 activities that varied in intensity from sedentary (eg, TV watchThg) to vigorous (eg., running) were collected While performing these activities, the girls wore two accelerometers, a heart rate monitor and a Cosmed K4h2 portable metabolic unit for measurement of [latin capital V with dot above]02. A random-coefficients model was used to estimate the relationship between accelerometer counts and [latin capital V with dot above]02. Activity thresholds were defined by minimizing the false positive and false negative classifications.
  • These cut points were originally defined for 30s epochs and were scaled up.

Mattocks Children 2005
 

• Reference Research
  • Mattocks C, Leary S, Ness A, et al. Calibration of an accelerometer during free-living activities in children. Int J Pediatr Obes. 2007;2:218–26.
• Special Notes
  • The children performed a series of activities (lying, sitting, slow walking, fast walking, hopscotch and jogging) while wearing an Actigraph and a portable metabolic unit. The sample was divided into a developmental and a validation group. Random intercepts models were used to develop a prediction equation in the developmental group. The equation was assessed in the validation group by calculating limits of agreement (actual minus predicted energy expenditure). Thresholds for moderate and vigorous activity were derived by refitting the energy expenditure model with V02 as the outcome.

Evenson Children 2008

• Reference Research
  • Evenson KR, Cattellier D, Gill K, Ondrak K, McMurray RG. Calibration of two objective measures of physical activity for children. J Sports Sci. 2008;26:1557–65.
• Special Notes
  • A calibration study was conducted to determine the threshold counts for two commonly used accelerometers, the ActiGraph and the Actical, to classify activities by intensity in children 5 to 8 years of age.  Thirty-three children wore both accelerometers and a COSMED portable metabolic system during 15 min of rest and then performed up to nine different activities for 7 min each, on two separate days in the laboratory. Oxygen consumption was measured on a breath-by-breath basis, and accelerometer data were collected in 15-s epochs

Pate Preschool 2006

• Reference Research
  • Pate, RP; Almeida, MJ; McIver, KL; Pfeiffer, KA; Dowda, M; Validation and Calibration of an Accelerometer in Preschool Children
• Special Notes
  • Thirty preschool children wore an ActiGraph accelerometer (ActiGraph, Fort Walton Beach, FL) and a Cosmed portable metabolic system (Cosmed, Rome, Italy) during a period of rest and while performing three structured physical activities in a laboratory setting. Expired respiratory gases were collected, and oxygen consumption was measured on a breath-by-breath basis. Accelerometer data were collected at 15-second intervals. For cross-validation, the same children wore the same instruments while participating in unstructured indoor and outdoor activities for 20 minutes each at their preschool.
  • Note that these cut points were originally developed using 15s as the original epoch length.  To obtain those cut points, reference the paper listed under Reference Research or divide the CPM values shown above by 4.
  • Note also that the Sedentary and Light cut points were added after consulting with K. McIver (author).  The interpretation was based on a visual inspection of the data.  Contact the author(s) for any further information.

Trost Toddler 2011
 

• Reference Research
  • Trost, SG; Fees, BS; Haar, SJ; Murray, AD; Crowe, LK; Identification and Validity of Accelerometer Cut Points for Toddlers. Journal of Obesity, August 2011
• Special Notes
  • Twenty-two toddlers (mean age = 2.1 years ± 0.4 years) wore an ActiGraph accelerometer during a videotaped 20-min play period. Videos were subsequently coded for physical activity (PA) intensity using the modified Children’s Activity Rating Scale (CARS). Receiver operating characteristic (ROC) curve analyses were conducted to determine cut-points. Predictive validity was assessed in an independent sample of 18 toddlers (mean age = 2.3 ± 0.4 years). 
  • These cut points were developed originally as 15s cut points.  They were scaled to their 60s equivalent with the author's permission

Troiano 2008

• Reference Research
  • Troiano, RP; Berrigan, D; Dodd, KW; Masse, LC; Tilert, T; McDowell, M; Physical Activity in the United States Measured by Accelerometer. Medicine & Science in Sports & Exercise, August, 2007
• Special Notes

  • These results were obtained from the 2003–2004 National Health and Nutritional Examination Survey (NHANES), a cross-sectional study of a complex, multistage probability sample of the civilian, noninstitutionalized U.S. population in the United States. Data are described from 6329 participants who provided at least 1 day of accelerometer data and from 4867 participants who provided four or more days of accelerometer data.

  • Though the full paper describes cut points for various ages, these cut points apply only to adults, 18 and older.
  • The sedentary level given here is assumed based on discussions with the authors and their affiliates.  This level is not exclusively called out in the paper and should not be used as a validated measure of sedentary activity in adults.  According to the paper, "For youth ages 6–17 yr, the age-specific criteria of the Freedson group, as published by Trost et al. (29), were used with thresholds for moderate activity of 4 METs and vigorous activity of 7 METs. This adjusts for the higher resting energy expenditure of children and youth (11,19). The specific criteria for each age are specified in the SAS code available at http://riskfactor.cancer.gov/tools/nhanes_pam (15)."  Those levels are as follows:

• Moderate thresholds (in CPM)
  • Age = 6: 1400
  • Age = 7: 1515
  • Age = 8: 1638
  • Age = 9: 1770
  • Age = 10: 1910
  • Age = 11: 2059
  • Age = 12: 2220
  • Age = 13: 2393
  • Age = 14: 2580
  • Age = 15: 2781
  • Age = 16: 3000
  • Age = 17: 3239
  • Age >= 18: 2020

• Vigorous thresholds (in CPM)
  • Age = 6: 3758
  • Age = 7: 3947
  • Age = 8: 4147
  • Age = 9: 4360
  • Age = 10: 4588
  • Age = 11: 4832
  • Age = 12: 5094
  • Age = 13: 5375
  • Age = 14: 5679
  • Age = 15: 6007
  • Age = 16: 6363
  • Age = 17: 6751
  • Age >= 18: 5999

You can find the current firmware versions by going to the website names below and choosing for the right device. 
http://www.actigraphy.nl/en/downloads 

Time Base Defined

During initialization, devices are given a "Start Time". That is, the time at which the device should start collecting data. The Start Time concept requires that the device itself be given a time base.  When a device is initialized, ActiLife sends the current time to the device to serve as this time base. The device contains a very accurate crystal oscillator which begins incrementing the time base. This acts as the device's on-board clock until the device is reinitialized. Although there is some clock drift expected, it is minimal due to the high accuracy of the on-board crystal oscillator.

Atomic vs Local

Beginning with ActiLife 6.5.0, the initialization screen offers users the ability to select between a time base from the local computer or an atomic clock. The choice can be toggled by clicking on the hyperlink to the right of the "Device Time" option as shown below:

 

When local time is selected, the devices will be programmed with the computers local time as the time base. When the atomic time is selected, ActiLife attempts to capture the time base from the United States National Institute of Standards and Technology (NIST) time servers list located at http://tf.nist.gov/tf-cgi/servers.cgi. ActiLife first attempts to make connection with one of five random NIST servers from the list given in the hyperlink. If a time is available, the computer's local time-zone offset setting is used to set the correct hour. The minutes and seconds settings captured directly from the NIST server are used to complete the time base. If none of the five random servers are available, the time base will default to the local time.

This feature is helpful when synchronizing data collection across multiple sites.

We are often asked if the Epoch Length researchers use will affect the results they get from the various analysis calculations in ActiLife. The answer is yes; all calculations for Adults in ActiLife are based on 60 second Epochs; all calculations for children are based on 15 second Epochs. All calculations scale linearly up or down from these values for other Epoch lengths. The generally accepted industry standard age ranges for classifying someone as adult or as a child are as follows:

• Infant = Less than 1 year old
• Toddler = 1 to 2 years old
• Pre-School = 3 to 5 years old
• Children = 6 to 18 years old
• Adult = 19 and older

To illustrate the difference that smaller Epochs can produce when scoring your data, we will compare two sample datasets; one that uses 10 second Epochs and one that uses 60 second Epochs (both samples are from the same dataset, just integrated at two different Epoch lengths).Smaller Epochs will produce a higher resolution dataset as there is more activity data for any given length of time (e.g. 5 minutes of 1 second Epoch data will yield 300 datapoints whereas 5 minutes of 60 second Epoch data will only produce 5). While you will get better resolution, the trade off in smaller Epochs is file size; smaller Epochs will create significantly larger files than larger ones.

The left side is 10 second epochs and the right side is 60 second epochs of the same data. For this example, we used the Freedson Combination (1998) EE formula and the Freedson Adult (1998) Cut Point classification (you can read more about the various algorithms available in ActiLife by going here). As you can see, for the cut point classification in 10 second epochs, there are 30 seconds worth of data in Vigorous activity levels and 30 seconds worth of Sedentary activity. In the 60 second epochs, there are 2 minutes worth of moderate activity. When it comes to kCals, for epochs less than 60 second seconds, we first scale the counts up to 60 seconds and then apply the Energy Expenditure (EE) formula. We then scale the result value back to the original Epoch value. As you can see, there is a significant difference in the resultant kCals. For this example, we used 100 kg as the mass for the EE algorithm.

There are similar differences in the METs, Bouts, and Sedentary Analysis due to the scaling.

We hope this helps to clarify how ActiLife treats different Epoch lengths. 

This tool will help with fixing DAT files which were downloaded/converted while using ActiLife 5.5.0. This tutorial is only for these files! In later versions the problems were solved.

Go to the following link to download 'DAT file fixer':
http://support.theactigraph.com/faq/dat-file-repair-tutorial

When the file is opened, it looks like this example:

1. Provide a back-up fro mall DAT-files which need to be fixed
2. Download these ( see upper link) and save at your desktop
3. Double click on the icon to start the program and choose 'select file(s)...'
4. Select the files which need to be fixed
5. Choose for 'fix file(s) to start the recovery process
6. The files will be replaced by fixed files like these:

What is it? 

• Your ANT Heart Rate Strap is a device worn in proximity to the heart that gathers electrical activity data to derive your heart beats per minute and/or higher resolution R-to-R data when combined with a wGT3X+

How do I use it? 

• Thoroughly wet both electrodes on the strap with water or electrode gel. It is important that the electrodes are sufficiently moist so that a good skin-to electrode connection is made.
• Snap one end of the Strap to one side of the Pod. Either end of the Strap can connect to either end of the Pod, but the Pod should have the logo facing up when in use.
• Wrap the Strap around your back and snap the free end to the free side of the Pod so that the Pod is located on your breastplate, just below your breasts. Adjust the Strap to create a snug, yet comfortable fit.
• Once in place, move within 5’ - 10’ of a wGT3X+ that has been configured to collect heart rate data.

It won't connect! What do I do? 

• If you do not get a reading, or the readings are erroneous, ensure that the electrodes are wet, have good contact with your skin and the that your Heart Rate Sensor is sufficiently snug. Your Heart Rate Sensor ‘sleeps’ with inactivity and ‘awakens’ with good electrical contact. A brief physical warm up may be required.

How do I pair it with my wGT3X+?

• As of ActiLife version 6.5.0, the wGT3X+ will simply automatically pair with the first heart strap it detects.  Support for pairing with a specific device will be added in a future version of ActiLife.

How do I change the battery?

• The battery is located behind a cover on the back of your Heart Rate Sensor Pod and can be replaced by placing a coin in the slot and turning the cover 1/4 turn counterclockwise. Replace with a 3 volt CR2032 battery.

When the LED is continuously lighted up whil unplugged, there is a possiblity that the device has a serious intern error. It is possible that the device cannot be initialized when plugged into the computer. The problem can be solved:

• Unplug the device and put it aside untill the LED is off. This means that the battery is totally empty. Then try to plug in the device again and see if the problem is solved. **
• Contact ActiGraph 

** This process can take several hours, depending of the battery status. With the LED on, the battery will be empty sonner, but it will still take one or several days.

In some cases, users may experience an error when updating to the latest release of ActiLife. The likely cause of this is a corrupt or malformed Preferences file that ActiLife is unable to import into the new version. You may see an error similar to the following screenshot:

To resolve this error, you will need to delete all previous preferences files and reinstall ActiLife. We will step through this procedure below.

Press and hold the Windows Key, then press the R key. This will open the Run Dialog box. If you are using Windows XP, enter "%USERPROFILE%\Local Settings\Application Data\ActiGraph\" (without the quotes) and click Ok. If you are using Windows Vista/7, enter "%LocalAppdata%\Actigraph\" (also without the quotes) and click Ok. This will open a Windows Explorer window where your User Preferences file(s) are stored. Simply delete the contents of the ActiGraph folder (do not delete the ActiGraph folder itself, only it's contents as shown below):

After you have deleted these folders, reinstall ActiLife according to the procedure outlined in the FAQ: "Installing/Reinstalling ActiLife 5.x/6.x"

If all went well, ActiLife should now open properly. Deleting these preferences files will reset ActiLife back to its defaults and you will need to re-enter any customizations that you have made for ActiLife (e.g. download locations, custom Cut Point sets, Date/Time Filters, etc) but you should now be able to open ActiLife as expected.

At the request of our customers, ActiGraph has compiled a number of sample Raw Data Files for use with ActiLife. These files will allow users to create AGD files of any Epoch length and familiarize themselves with the various analysis tools in ActiLife. To keep these to a reasonable download size, these Raw Data Files have been split into four files:

• GT3XPlus-RawData(1-20).zip (169mb) - This Archive contains Raw Data in 1, 2, 3, 4, 5, 10, 15, and 20 day lengths. This was collected using a GT3X+ Device.
• GT3XPlus-RawData(30).zip (91mb) - This Archive contains a 30 day sample Raw dataset. This was also collected using a GT3X+ Device.
• ActiSleepPlus-RawData(1-20).zip (287mb) - This Archive contains Raw Data in 1, 2, 3, 4, 5, 10, 15, and 20 day lengths. This was collected using an ActiSleep+ Device.
• ActiSleepPlus-RawData(30).zip (148mb) - This Archive contains a 30 day sample Raw dataset; This was collected using an ActiSleep+ Device.

Use of the GT3X+ datasets will allow access to all analysis tools available to GT3X+ accelerometers. Using the ActiSleep+ datasets will provide access to the additional tools only available for the ActiSleep+ devices (e.g. AutoSleep Scoring, Clinical Reports, Periodic Limb Movement (PLM), etc). This is actual activity data collected by an ActiGraph employee wearing ActiGraph activity monitors.

Please note; in order to use the Data Analysis tools in ActiLife, you must be using the Full Edition of ActiLife; the tools are not available in the Lite Edition.

http://support.theactigraph.com/faq/gt3x-data-recovery-wizard-tutorial

The instructions for the GT3X+ Recovery Wizard are described below:

The newest from the GT3X+ data recovery wizard is out! This version adds the possibility to fix data from firmware version 01.07.00 after a period of power failure. These devices are identificiable by the fact that when connecting to ActiLife, there was a battery fail and no data was available.

Devices with version 01.05.00 with corrupt files can also be fixed with this application. To prefent data loss this application cannot initiate, update or remove data. When you are convinced that data has been correctly fixed, the ActiLife application can be used to update the firmware. This process will remove all data from the device. So be sure that the data is correctly fixed!

For further questions you can contact us! 

With the release of ActiLife 6.5.x, the firmware file format was changed to allow for additional functionality with the latest revisions of GT3X+, ActiSleep+, wGT3X+, and wActiSleep+ devices. However, because of this change, ActiLife can no longer automatically update from much earlier revisions of firmware. You are also not able to manually update from these versions to the latest release. ActiLife automatically tries to update the firmware to newer version when you initialize the device, and because this older firmware is unable to be automatically updated, ActiLife will get caught in an Update Loop and never complete the initialization.

To resolve this issue, users simply need to update to an intermediate build, and then from there, you will be able to update the firmware to the current releases. This ONLY affects devices with serial numbers that begin with CLE, MRA2, and MRA3 that have Firmware Revision 1.0.0, and if you are using ActiLife 6.4.5 or earlier. You must have ActiLife 6.5.1 or greater installed to perform this procedure; Devices with these serial numbers are not compatible with earlier versions of ActiLife. It does not affect any other devices.

To perform this procedure, first download and save the 1.02.07 firmware to your computer. You can find this firmware file at the end of this article. Make sure that you have installed at least ActiLife 6.5.1 or greater. Once ActiLife is installed, connect your device to your computer via it's USB connection. It is recommend that you connect directly to the computer and bypass any USB hubs that you might have been using. Once the device is visible in the grid, click on Communication, then Advanced, then Update Firmware From File:























You will then see a browse dialog box. Simply browse to the location where you saved the attached firmware file and double-click on it to begin the update process. You will then see a series of Status updates in the devices grid; "Updating" and then "Finished Update". The device will then disappear from the grid and should reappear after a few seconds. You will notice that the Status now says "Finished Updating" and the firmware on the device is now 1.2.7.











































You should now be able to initialize your device and the firmware will automatically update to the latest release with no further intervention required. To verify this, simply initialize the device and if all goes as expected, when the device reappears in the grid, you will see that the firmware has once again updated, only now, it will be the most recent release (2.0.0 in this instance).

 

Firmware:FAQ_firmware 01.02.07

ActiLife 6.5 and later combined with wGT3X+ devices running firmware 3.0 or newer are capable of providing measurements of Heart Rate R-to-R intervals. 

Collecting Heart Rate Data
Heart rate data is captured from the wireless ANT+ enabled heart rate chest strap and is transmitted in real time to the wGT3X+.  In order to capture heart rate data, the following must occur:

1. Initialize a wGT3X+.  Be sure to enable "ANT+ Wireless" and "Heart Rate".  Setting a PIN code is optional.  The PIN code is required when communicating with the wGT3X+ from one of ActiGraph's mobile applications.

2. Position the ANT+ enabled heart rate strap around the chest area.  Instructions for wearing the heart rate strap are available here.
3. The wGT3X+ will pair over the ANT+ wireless channel with the first heart strap that it detects and will lock to that heart strap.  As of ActiLife 6.5.0, there is no way to pair a single heart strap directly to the wGT3X+.  This feature is coming soon.

• If the wGT3X+ loses connection with the heart strap, it will begin searching immediately for another one.  If no heart strap is detected, the wGT3X+ will wait 15 seconds before performing another scan.  If no heart straps are detected, it will wait another 30 seconds before performing another scan.  If no heart straps are detected, it will wait another 60 seconds before performing another scan.  If no heart straps are detected after that waiting period, the wGT3X+ will search for nearby heart straps once every 2 minutes.  This method prevents the wGT3X+ from completely depleting its battery life if the heart strap is no longer present.

4. Wear the wGT3X+ and the heart strap for the desired amount of time.

Downloading Activity and Heart Rate Data
Connect the wGT3X+ to the computer running ActiLife and download the data.  The *.agd file can be created at time of download.  Be sure to check the "HR" option during download to ensure that the raw Heart Rate data collected on the wGT3X+ gets exported to the *.agd file for use in ActiLife.

Alternatively, the *.agd file can be created after download by clicking "finished downloading" from the device list or later by simply opening the raw *.gt3x file that is generated at time of download.

2. The resulting *.agd file will contain basic beats per minute data for each epoch.  This can be viewed by opening the *.agd file in ActiLife (simply double-click on the *.agd file).  

Extracting R-to-R Heart Rate Data
1. To extract more detailed heart rate data (R-to-R values), either a) select the "Heart Rate R-R" option from the File->Import/Export menu in ActiLife or b) select "Export Heart Rate R-R" from the AGD File Viewer export menu as shown below 

2. Select the *.agd file that contains the heart rate data you wish to examine.  A *.csv file will be created in the same directory.

Understanding the R-to-R Heart Rate Data
The R-to-R heart rate data can be examined in Microsoft Excel®. There are three columns:

• Timestamp - the long date and time (with millisecond resolution) at which a heart beat was detected
• Time since last beat (milliseconds) - the number of milliseconds since the last beat
• Beat count - A consecutive beat counter that resets at 255.  This helps when examining missing beats.

• PLMI (per hour) - PLMI stands for Periodic Leg Movement Indices.  This value represents the number of qualifying periodic leg movements per hour, averaged over the entire sleep period.
• Minutes of PLM Kicks - The total number of minutes of "kicks" or activity spikes that exceed the onset threshold AND the total minutes of consecutive "kicks" thereafter that exceed the decay threshold.
• Number of PLM Kicks - The number of "kicks" or activity spikes that exceed the onset threshold AND the number of consecutive "kicks" that exceed the decay threshold.
• Number of PLM Bouts - A count of the number of bouts of PLM that occurred during the selected sleep period.
• Average PLM Bout Length (min) - The average length, in minutes, of all of the PLM bouts for the selected sleep period.
• Minutes of PLM Bouts - The total time, in minutes, of PLM bouts.  This time is different from the PLM kicks because kicks do not necessarily qualify as valid PLM.
• Avg PLMS Intensity - The average intensity of the PLM during Sleep of all of the PLM bouts.  Essentially, this is the duty cycle of all PLM bouts.  Avg PLMS Intensity = total minutes of valid PLM / total PLM bout length.

Two new advanced data exporting options were introduced in ActiLife 6.5.0

Exporting Only Valid Wear Time
Following a Wear Time Validation analysis, valid wear time can be exported directly to *.csv format for further analysis outside of ActiLife.  
IMPORTANT: While it is now possible to export wear time, it is advisable to simply work with the *.agd file in ActiLife.  Keep in mind that Wear Time Validation simply flags non-wear epochs in the *.agd file, thus allowing ActiLife itself to perform further analysis which do not require any type of exports.
To export valid wear times, perform a normal Wear Time Validation process by selecting the desired settings.  Click the "Details" button next to the file from which you would like to export the valid wear time as shown below.

Clicking the "Export" button will reveal two options for exporting data from the Wear Time Validation Detail: "Day and Wear Information" and "All Wear Time Epochs"

Exporting Day and Wear Information
Choosing this option will export a *.csv of the Days and Wear/Non-Wear data that appears in the left-hand area of the Wear Time Validation details view.  This includes a summary of Dates, Wear/Non-Wear Minutes, Wear/Non-Wear % and a detailed breakdown of the wear/non-wear time periods for the entire dataset.  Simply choose a save location and a file name to create the export.

Exporting all Wear Time Epochs
Choosing this option will generate an export of all wear time epochs.  This includes, for a 60s epoch file, a minute-by-minute breakdown of all data for valid wear times for the entire dataset.  Simply choose a save location and a file name to create the export.

Exporting Calculated Kcals and MET Rates per Epoch
Following a Data Scoring analysis, users may export calculated Kcals and MET rates.  Click the "Details" button to the right of the file to open the "Data Scoring Details" view as shown below:

The Custom Sleep Scoring algorithm builder allows users to create their own custom sleep scoring algorithm based on the techniques used to create the Sadeh and Cole-Kripke algorithms.  The custom sleep scoring algorithm builder utilize a sliding window technique to flag epochs as "Sleep" or "Wake".  The results will be used to create sleep efficiency scores and other details about sleep.  Read more about the sleep scoring tool in the ActiLife 6 Users Manual.

From the 'Options' menu, navigate to Calculations then 'Sleep Scoring'.  In the Algorithm dropdown box, select "Create New".  In the form that appears, enter in a name for the new custom algorithm.  Then select the number of epochs to use before and after the current epoch (weighting) in determining the "Wake" or "Sleep" status of the current epoch.  In the boxes that appear below the epochs, set a weighting value.  For example, setting a value of 0.25 on epoch-2 will multiply that epoch value by 0.25 and will add it to the other epoch weights and compare to the "Wake Threshold" to determine how epoch-0 will be flagged ("Wake" or "Sleep").  

Use the "Count Parameters" section to set the "Wake Threshold" and "Epoch Length Required" (the epoch length that the algorithm should expect to work with - this will cause ActiLife to reject files with epoch lengths that do not match that value.  The "Set Maximum Value of Count" option limits the epoch value during the multiplication process.  For example, if epoch-2 is happens to be 2024 counts (for instance), the Maximum Value option is set to 300, and the epoch-2 weight is set to 0.25, the resulting weight of that epoch will be 0.25 * 300 = 75.  

The "Sleep/Wake Formula" at the bottom of the algorithm builder automatically updates to show the resulting formula.  Epochs that produce values that are greater-than-or-equal-to the threshold are flagged as "Wake" while epochs that are less-than that threshold are flagged as "Sleep."

The serial numers of the GT3X+ (nonwireless) starts with CLE1. The serial number for the wGT3X+ (wireless) starts with CLE2.

Furhtermore, the name 'wGT3+' is present on the rubber material of the wireless ActiGraph.

The Wear Time Validation (WTV) tool excludes non-wear periods from datasets for further analysis.  Non-wear periods are characterized as periods of complete inactivity. While the non-wear detection algorithm can be customized in the WTV tool, occasionally sleep periods may be inadvertently classified as non-wear periods because of their low level of activity.

ActiLife 6.5.0 introduces the option to exclude sleep times from WTV, in effect flagging all sleep periods as valid wear time.  Begin by flagging the Time-in-Bed and Time-Out-of-Bed (TOB) times for a given dataset from the Sleep Analysis tab.  Here's how:

Graphically Flag Bed Times
To graphically flag TIB and TOB times, left-click on the approximate TIB time (the time the patient went to bed) for any given night.  Use the left-right keyboard buttons to tweak the time on a minute-by-minute basis.  Next, right-click anywhere on the graph and choose "Accept New In Bed Time Here" as shown below.

Repeat this process for the TOB.  Right-click and choose "Accept Out Of Bed Time" once the desired time is selected as shown below.  

This will create and score the time period for sleep efficiency and will store those times in the *.agd file.  Repeat this process for all bed times until all of the TIB/TOBs have been recorded.

Typing in Bed Times
Bed times can also be keyed in by simply clicking "Add Sleep Period" to the right of the Sleep Analysis tab.  Select the In Bed Date and Time and Out Bed Date and Time.  Click "Accept" to lock in the sleep period.  Repeat this process until all sleep times are entered.  A screenshot of the manual bed time entry tool is shown below.

Automatically Estimating Bed Times
To quickly estimate bed times, simply click the "Auto Score" button in the upper right-hand corner of the Sleep Analysis tool.  This will delete all of the files existing TIB/TOBs and will perform an algorithm to estimate the bed times.  These times can be tweaked by clicking the "edit..." link to the right of each bed time if desired.  NOTE: The Auto Score feature is only available for ActiSleep and ActiSleep+ devices.  This feature can be turned on remotely for GT3X, GT3X+ and wGT3X+ devices for a small fee.  Contact sales@theactigraph.com for more information.

Deleting Bed Times
Bed times can be deleted individually or all at once.  To delete individual bed times, simply select the desired sleep period by clicking on the pink area in the graph or by selecting the sleep period from the list.  Click the "Remove Period" button below the bed time list on the right.  Alternatively, simply right-click on the sleep period on the graph and choose "Delete Current Sleep Period".  To delete all bed times, right-click on a graph away from a sleep period and choose "Delete All Sleep Periods In This Day" or "Delete All Sleep Periods For The Dataset" as shown below.

Excluding Sleep from Wear Time Validation
To exclude sleep times from WTV, simply load the *.agd file that contains predetermined sleep times into the WTV tool by dragging and dropping onto the WTV tool or by selecting the file through the "Add Dataset(s)" button in the WTV tab.  Check the "Mark Sleep Periods as Wear Time" and run the WTV tool as normal.  Sleep times will be flagged as valid wear time regardless of their activity levels.

To upgrade your virtual trainer account from 'Free' to 'Pro', you can follow this instructions:

1. Log in at your virtual trainer account:  http://app.actitrainer.com/
2. At the right of your screen you'll find a link to 'Profile'
3. Choose for the 'Sign Up For Pro!' link 
4. Select a way of paying and follow the instructions on the screen
5. When you ran through the procedure, you account will automatically transformed into the 'Pro' version 

To delete your account online, you can follow the next steps:

- Login at your account at http://app.actitrainer.com
- At tje right side of the screen you'll see a link to ‘Profile’
- Go to your ‘profile editor’ page and choose for ‘change plan’.



- On the next page ‘change subscription plan’, you will find a link to ‘cancel subscription’, click on this.



- Follow the instructions on the screen and complete the deleting process. 

You can convert AGD and GT3X+ files to one or more of the following: DAT, CSV and AGD. ActiLife alsohas other features for converting these files to specialized CSV file or other AGD files.
1. To begin, start byclicking on the "File" menu in ActiLife.
2. Then click on "Import/Export" to get a large selection of conversionoptions!
3. Select the option you wish to conver FROM and TO (ie AGD -> CSV).

Upon your choice, youwill be given the option to select one or more files to convert. You can convert many files at once! You will begiven a progress indication once you have submitted your file for conversion. Once complete, there will be a newset of files (along with your originals) that are now converted!

Note: In order to convert ActiLife AGD files to other formats, you must be using the FULL edition of ActiLife.

The Periodic Leg Movement (PLM) tool allows users to detect and score periodic leg movements in patients with PLM disorder or restless leg syndrome.  Note: The PLM tool is only compatible with data collected from the legacy ActiSleep or the new ActiSleep+ monitor devices. 

Initializing Devices
To use the tool, initialize an ActiSleep monitor through the devices tab to start collecting data at the desired time.  (Info about ActiSleep battery life) ActiSleep+ devices only have one option for initialization (30Hz).  Legacy ActiSleep devices should be initialized to collect data at 1s epochs.  During initialization, ActiLife gives users the option to print a sleep diary.  The sleep diary can be used by patients who choose to wear the devices for several nights in their own homes or for doctors who are logging multiple patients' sleep behaviors.   Patients should be instructed to use the sleep diary to keep track of the time in bed (TIB) and time out of bed (TOB) for purposes of analysis.

Attach the ActiSleep monitor to the top interior of the patient’s left and right feet around the ball of the foot as shown in the image below.  This can be accomplished with bandage wrapping or with the straps provided with the ActiSleep monitoring package.  Patients should wear the device during the entire duration of their total time in bed.

Downloading Devices

After patients have worn the devices for the desired number of sleep periods, collect the devices and download them using the Devices tab.  Legacy ActiSleep monitors will produce an *.agd file on download.  This file can be used for scoring PLM in the PLM tab.  ActiSleep+ devices will create a *.gt3x file on download.  This file contains the raw acceleration data collected by the devices and needs to be post-processed (i.e., converted to *.agd format) prior to PLM scoring.  This can be accomplished by choosing “Create AGD File” from the download dialog as shown in the figure below.  Select 1s epoch and 3 axes.  The other options are optional, but “Low Frequency Extension” should not be selected.

• Add Period Using Bed Times– Use this option to manually enter in times as recorded by the doctor or patient on the sleep diary.
• Import from Separate AGD File– Use this option to import sleep times from another AGD file that already contains the same sleep times.
• Enter Period Graphically- coming soon)

• Onset Threshold: The threshold (in counts-per-epoch) at which PLM movement detection starts
• Decay Threshold: The threshold (in counts-per-epoch) at which PLM movement detection ends.

In order to install ActiLife properly, you must have administrative rights on the computer on which you wish to install ActiLife and due to a recent Microsoft update, you may have to allow the installer to have full permissions to install all required components. First, please make sure you have downloaded the latest installation executable for ActiLife 5.x/6.x. You can get these from the following location:

ActiLife 5.x - http://www.actigraphy.nl/en/downloads
ActiLife 6.x - http://www.actigraphy.nl/en/downloads

Please note: ActiLife MUST be installed locally. Roaming Profiles or Profile Folder redirection are NOT supported.

Also Note: ActiGraph is no longer able to offer support for ActiLife software prior to version 6.0, released in February 2012. This policy, effective December 31, 2012, was implemented to allow our technical and development teams to focus exclusively on supporting the new and improved ActiLife 6 platform. Those customers operating ActiLife versions 4 and 5 will be able to continue to use their software, however ActiGraph no longer provides assisted support, program updates, new bug fixes, automatic device firmware updates and online technical content updates. In order to reduce the risk of potential device and/or data issues related to outdated software, we strongly  encourage our customers to upgrade to ActiLife 6. You can upgrade by visiting our online store (click here) or by contacting our Sales Department by emailing them at sales@actigraphy.nl

You will need to download and save this file to your computer. Once it is downloaded, right-click on the file and choose "Properties". On the General tab, near the bottom of the window, you may see a heading that says "Security" with a button next to it that says "Unblock". It should look similar to this screenshot:

 

Click on the Unblock button and then click Apply and then Ok. If you do not see this button, the security update in question has not been installed and you may proceed with the next step of the installation.

Now right click on the file one more time and choose "Run as..." (for Windows XP) or "Run as Administrator" (for Windows 7/Vista). If prompted for a user account, choose Current User and Uncheck the box below that that says "Protect my computer...". Then click Ok again. This will allow the installer to properly install of the necessary components for ActiLife to work. If you do not perform these steps, it may appear that ActiLife has successfully installed but critical components will have failed to be installed.

If you see a prompt like the one below, asking to install drivers, tick the box that says "Always trust software from ActiGraph" and click the Install button.

 

After the installer has completed, uncheck the box that says "Run ActiLife" and click Finish. Restart your computer to ensure that any required services start correctly. Once Windows has booted back up to the Desktop, you should be able to open and use ActiLife. If this is a new installation of ActiLife, you will be prompted to enter your Product Key when you open it for the first time.

Please note: running ActiLife inside of a virtual environment like VMWare, VirtualBox, or Parallels is NOT supported (BootCamp on a Mac should work fine as you are technically booted into a native Windows environment when doing that). At this time, ActiLife requires that you are using Windows in a native, non-emulated configuration.

If you do not have your Product Key or if you need any further assistance, please visit our support portal at http://www.actigraphy.nl/en/contact. From there, you can view many helpful FAQ's and Troubleshooting Articles; log in to check any existing Support Requests; or create a new Support Ticket if you are unable to find a solution. You can also email us at support@actigraphy.nl.

All of ActiGraph's devices** will indicate that they are fully charged (the battery is at 100%) by displaying asolid LED (light) while attached to either a computer's USB port or powered USB hub. This light could be eitherwhite or green, depending of the device.

Please refer to the follow FAQ 'what does a blinking LED mean?' for more informationon the LED.

** This excludes the ActiTrainer device, which displays a real-time battery indicator within itsdisplay.

Users can set date and time filters within the Data Scoring tool.  These filters allow users to perform their analysis on specific dates and/or periods of time.  Date and time filters are stackable, meaning that users can create multiple overlapping filters (e.g., Weekends and Mondays).  To create a new filters, click the "+" icon in the Date and Time Filters section of the Data Scoring tab as shown below.

• Filter Name - The name of the filter as it will appear in the filter list.
• Date Options - The specific day or groups of days (weekends/weekdays) that the filter should include.
• Start/Stop Times - The times of day the filter should include.

Click Save.

 

A list of filters will appear as they are added.

To exclude a filter from the current analysis, uncheck the box to the left of the filter.  Checked filters apply to all files in the Data Scoring grid and area also applied when exporting the data to Excel (using the export option on the bottom right).  ActiLife remembers filters, even when the application is closed and reopened.

The GPS tab in ActiLife 6.3.0 and later gives the user the option to correlate their epoch level actigraphy data with time-stamped GPS data.

Supported GPS devices and input formats include:

• Garmin (*.tcx)
• Qstarz (*.csv)
• iTrail (*.csv)

In addition, the source GPS data will be saved in the actigraphy data file (*.agd).  This eliminates the need to re-import the GPS data for future re-calculations. The correlation process does not affect the actigraphy data in any way.

Using the GPS Correlator
To begin, click the "Select Dataset" button and select an *.agd file that contains epoch-level actigraphy data from an ActiGraph device that was worn simultaneously with a GPS logging device.  Any epoch level greater than 1 second is allowed.  If this file contains GPS correlated data, the GPS correlator tool will give the option to delete or select another GPS dataset to replace the existing GPS data.

In the GPS Data section, select a device type and browse to the GPS file that contains the time-stamped GPS data.  ActiLife will prompt with an alert if this data does not align with actigraphy data in the *.agd file selected above.

In the results section, select the desired output(s).  "Create a CSV correlation output" will create a CSV file that contains both the epoch level actigraphy data and GPS data in separate columns, correlated by timestamps.  The "Create an intensity map (KMZ) output" option will create a KMZ file which contains correlated GPS data points and cut point intensity levels (see image below).  Users can open the KMZ file in various programs including Google Maps.  Select the desired cut point set (defaults in ActiLife - read more here) or create a new custom set by clicking the "edit" link to the right of the cut point selection combo box.  Checking the box "Open with Google Earth" will automatically load the intensity map in Google Earth after the correlation is complete (Google Earth must be installed on the same computer as ActiLife). 

A word about outputs

The GPS correlator produces potentially three (3) outputs:

1. The dataset (*.agd file) will be modified to include ALL of the GPS data.  For example, if GPS data and actigraphy are at different resolutions (e.g., 10s epochs and 1s GPS data), the correlator tool will insert ALL of the GPS data into the *.agd file.  The original actigraphy data will not be affected.  ActiLife will properly handle the file regardless of the presence of the GPS data.  The GPS data can be deleted from the *.agd file at any time.
2. If selected, a CSV output will be generated.  Epoch-level actigraphy data always takes priority during the correlation process.  If present, excess data from the higher resolution GPS file is discarded.  For example, a 10s epoch actigraphy file correlated with a 1s epoch GPS file will result in a 10s epoch CSV file.  The correlator tool will use the closest matching GPS timestamp to correlate with each 10s epoch actigraphy count.  In addtion, GPS data points that are not within the timespan of the first and last activity data point are not output.
3. If selected, a KMZ output file will also be generated.  Similar to the generation of a CSV file, the KMZ output will be driven by the epoch-level actigraphy resolution.  A 10s epoch actigraphy file correlated with a 1s epoch GPS file will result in a 10s resolution KMZ file; excess GPS data will be discarded in this file.

Sedentary Analysis Description
The Sedentary Analysis feature identifies bouts of low activity in *.agd datasets.  Following Wear Time Validation, this feature will identify details about a subject's sedentary behavior (rather than identifying non-wear as sedentary time).  When enabled, this feature yields following outputs in the Data Scoring tool:

• Summary Screen
  • Total Sedentary Bouts - the number of sedentary bouts detected in the entire dataset
  • Total Length of Sedentary Bouts - The total sedentary time detected in the entire dataset.  
  • Max Length of Sedentary Bouts - The length of the longest sedentary bout in the entire dataset
  • Min Length of Sedentary Bouts - The length of the shortest sedentary bout in the entire dataset
  • Daily Average of Sedentary Bouts - The Total Length of Sedentary Bouts divided by the total valid days in the dataset
  • Total Sedentary Breaks - The total number of breaks in sedentary activity for the entire dataset.
  • Total Length of Sedentary Breaks - The sum of all the times between sedentary bouts in the dataset.
  • Average Length of Sedentary Breaks - An average of all of the times between sedentary bouts (Total Length of Sedentary Breaks/Total Sedentary Breaks)
  • Max Length of Sedentary Breaks - The length of the longest sedentary break in the entire dataset
  • Minimum Length of Sedentary Breaks - The length of the shortest sedentary break in the entire dataset
  • Daily Average of Sedentary Breaks - The Total Length of Sedentary Breaks divided by total valid days in the dataset

An example Summary Screen is shown below:

 

• Details View - After clicking "Details" button
  • Total Sedentary Bouts - the number of sedentary bouts detected in the specified day/hour
  • Total Length of Sedentary Bouts - The total sedentary time detected in the specified day/hour
  • Max Length of Sedentary Bouts - The length of the longest sedentary bout in the specified day/hour
  • Min Length of Sedentary Bouts - The length of the shortest sedentary bout in the specified day/hour
  • Daily Average of Sedentary Bouts - The Total Length of Sedentary Bouts divided by the total valid days in the specified day/hour
  • Total Sedentary Breaks - The total number of breaks in sedentary activity for the specified day/hour
  • Total Length of Sedentary Breaks - The sum of all the times between sedentary bouts in the specified day/hour
  • Average Length of Sedentary Breaks - An average of all of the times between sedentary bouts (Total Length of Sedentary Breaks/Total Sedentary Breaks) in the specified day.
  • Max Length of Sedentary Breaks - The length of the longest sedentary break in the specified day/hour
  • Minimum Length of Sedentary Breaks - The length of the shortest sedentary break in the specified day/hour

A sample "Details" view is shown below:

 

Sedentary Analysis Setup
To setup and run a sedentary analysis, check the box beside the "Sedentary Analysis" option in Data Scoring and click "edit..." to define sedentary analysis parameters.  A sample window and a description of the parameters is given below.

 

• Bout Name - Uneditable
• Length
  • Minimum - The minimum allowable amount of consecutive time that can be considered a sedentary bout
  • Use Maximum - (optional) The maximum allowable amount of consecutive time above the minimum that can be considered a sedentary bout
  • Drop Time - The tolerance, or allowable amount of total time (not consecutive) that can break a sedentary bout.  
• Count Levels
  • Minimum - The minimum count level to be considered a sedentary bout
  • Use Maximum - (optional) The maximum count level to be considered a sedentary bout
  • Use Vector Magnitude - This option uses the Vector Magnitude of all three axes when considering the count level values defined in the Minimum and Maximum.
  • Ignore First Sedentary Break of Each Day - If this option is checked it will not factor the time between the first sedentary bout of the day and the last one on the day before when calculating stats. So if you have a sedentary bout from 9:00pm to 9:30pm one night and then the next morning you have one from 9:30am to 10:00am, ActiLife does not assign the time from 9:30pm to 9:30am as a sedentary break.

Example: Consider a 60s epoch file.  If a 10 minute minimum is set, a drop time of 2 minutes is entered, and a maximum count level is 99, the following sequence would be considered a sedentary bout: 

• 10:01am - 50 counts
• 10:02am - 150 counts
• 10:03am - 0 counts
• 10:04am - 0 counts
• 10:05am - 0 counts
• 10:06am - 0 counts
• 10:07am - 500 conts
• 10:08am - 40 counts
• 10:09am - 30 counts
• 10:10am - 0 counts

Epoch time episodes refer to the data within the epoch according the time stamps below. You'll see an example where data in 15 seconds epochs are collected, started at 9:00:00

Data from 9:00:00 – 9:00:14 will be marked as 9:00:00
Data from 9:00:15 – 9:00:29 will be marked as 9:00:15
Data from 9:00:30 – 9:00:44 will be marked as 9:00:30
Data from 9:00:45 – 9:00:59 will be marked as 9:00:45
Data from 9:01:00 – 9:01:14 will be marked as 9:01:00
Data from  9:01:15 – 9:01:29 will be marked as 9:01:15

Data is stored on ActiGraph products in one of two ways: either post-filtered and accumulated or raw.

Postfilteredand accumulated data is typically stored in time buckets referred to as “Epochs.” On ActiGraph devices,“Epochs” are whole-second numbers (e.g., 1s, 5s, 60s, 120s, etc.). For each epoch interval, data samples takenfrom the accelerometer inside the device at a rate of 30Hz are first filtered then accumulated before being storedin memory. For example, a device set to collect 60 second epoch data will store 30 × 60 = 1800 accumulatedsamples for each enabled axis on the device every 60 seconds.

GT3X+ devices do not store postfiltered/accumulated data - only raw data.

Devices can be set to store data in raw mode by selecting a samplefrequency (12Hz or 30Hz) rather than a whole-second number in the “Epoch” dropdown during the initializationprocess. When raw mode is set, devices store raw (non-filtered/accumulated) accelerometer data (in units ofgravity – G’s) directly into memory at the selected rate (12Hz or 30Hz). When raw mode is enabled, nonaccelerometerchannels (steps, inclinometer, and lux) are disabled. Data stored at 12Hz is stored at a resolutionof 1/10 (0.1) G (unit of gravity). Data recorded at 30Hz is recorded at a resolution of 1 milli-G (Plus or minus3G limit). The GT3X+ product stores data in raw mode at all times. Data collection frequency is variable (30Hzup to 100Hz in 10Hz increments) and can be set during initialization.

Similar to other devices, the GT3X+ storesraw accelerometer samples in units of G’s at the indicated frequency. However, unlike other devices it alsostores lux (ambient light) samples once per second at all times. The GT3X+ has a dynamic range of +/- 6 Gs.

ActiGraph GT3X+, ActiSleep+, wGT3X+, and wActiSleep+ devices are designed to preserve battery life when the device is idle (not experiencing movement). This feature, often referred to as "Idle Sleep Mode" can be enabled or disable upon initialization.  Below is a description of the device's behavior with Idle Sleep Mode enabled and disabled.

Disabled
When Idle Sleep Mode is disabled, the device collects data at the desired sample rate without entering any idle state.  Each sample stored in the devices memory represents the exact reading from the internal accelerometer for the entire duration of data collection.  Chart 2 in the help thread Battery Life vs Memory Recording Limits for GT3X+ series shows the expected battery life for GT3X+ devices under this condition (wGT3X+ battery life information coming soon).  

Enabled (Default)
Data Collection
Devices with Idle Sleep Mode enabled will enter a sleep or low power state after experiencing 10 seconds of inactivity (fluctuation on the accelerometer < +/- 40 milliGs).  After entering this low power state, the device checks once every second to determine if the unit has moved.  While in sleep mode, the last sampled accelerometer value is written into memory at the device's preset sample rate.  [For example, a device set to sample at 30Hz would store the last known accelerometer reading 30 times every second; the device would then wake up and check for movement.  If no movement were detected, this pattern would continue.  Otherwise the unit would exit sleep mode (i.e., "wake up") and continue sampling in normal fashion.]  After filtering (conversion to *.agd), this data becomes 0 counts since no movement is detected during this time period.

LED Functionality
During low power state, the LED will stop flashing on devices that have the "flash LED during data collection" feature enabled.

LUX Data Collection
Because lux data is recorded every one (1) second regardless of the selected frequency of actigraphy data collection, lux data is continually stored even during low power state.  That is, lux data storage is unaffected by the Idle Sleep Mode functionality

Idle Sleep Mode has no effect on the wireless functionality for wGT3X+ or wActiSleep devices.  More information on the estimated power savings and battery life impact of Idle Sleep Mode can be found at Battery Life vs Memory Recording Limits for GT3X+ series.

****Please note that this only applies to version of ActiLife prior to ActiLife5.****

 "Address Pointer = 0"means that there is no data on the device. In other words, the device was:

1. Never initialized or initializedimproperly
2. The device is still in its "Delay Mode"

You can check the state of the device by looking at the reportstatus view (ActiLife version older than 4.0.0 use Options -> Device Information in the menu bar). The information displayed here can help determine the reason for this error. Here are a few possibilities that maycause the software to display this error:

- The device was initialized as usual, but then an attempt was made todownload data from the device before the device could start. This indicates that that device is still in "DelayMode." This mode is the time period between initialization and the start time/date you specified during theinitialize setup. You must wait until the device has had adequate time to begin collecting data before attempting todownload from it.

- The device's firmware was upgraded and then there was an attempt to download data beforethe device was initialized. Upgrading the firmware on the device ensures reliable functionality, however thisprocess erases all data on the device and sets the device in "Halt Mode." This is a special mode that prepares thedevice for initialization upon next use. In order to use this device again, you must initialize the unit.

Here is a viewif the device was never initialized (showing both old view and new):




Notice the "Modes" section of the aboveview. The "Initialized" check box is clear indicating that this device was never initialized. This most likely resultedfrom a recent firmware upgrade.

This view is correct if the device's firmware was recently upgraded. Also noticethe "Subject Name" under "General Device Information." It states "Not Initialized" to help indicate the device'scurrent state. Here is a view of a device that is still in "Delay Mode": Notice the "Modes" section.




The device isinitialized, but it is still in delay mode. The device is in this mode because the start date/time has not yet elapsed.(For this example, the device was initialized at 7:00:00 AM on December 29, 2008. Since the device is in thismode, it will not take any data until that start time occurs.

Yes, this is possible. Data can be uploaded to an ActiSleep account in .dat format by using 'ActiSleep Client Application'  (http://support.theactigraph.com/product/ActiSleep-software). Important is that this data will override all data on that ActiSleep account in .dat format.

ActiLife requires that the Microsoft DOT Net framework, v3.5, SP1 be installed on the computer where you will be installing ActiLife. This is a free download and should be included in Windows Vista/7 but may not be installed on Windows XP. You can find this at the following location:

Web Installer (only downloads minimum components. Much smaller download):
http://www.microsoft.com/download/en/details.aspx?id=22

Full Installer (larger download. Necessary for computers without internet access):
http://www.microsoft.com/download/en/details.aspx?id=25150

You may also want to go ahead and install the DOT Net 4.0 and 4.5 framework as well; these can all be installed side by side on the same computer. You can find these at the following locations:

DOT Net 4.0:
Web Installer: http://www.microsoft.com/en-us/download/details.aspx?id=17851
Full Installer: http://www.microsoft.com/en-us/download/details.aspx?id=17718

DOT Net 4.5 Update:
Web Installer: http://www.microsoft.com/en-us/download/details.aspx?id=30653

After installing this requirement, restart your computer and try installing ActiLife again.

The seize of the bought account will define the amount of patients/subjects you can save at your account. You can save unlimited information within the account under each patient/subject.

In the event that any device depletes its battery below the standard operating charge (this varies from device todevice), the device will safely store all collected information and shutdown. You can still safely download thedata from the device if this happens. Be sure to fully charge your device before using it again!

Yes, everybody who owns a user name and password for the ActiSleep account can log in and take a look at the data. To be able to upload the data from different computers, the ActiSleep software needs to be installed on all computers. 

Note: HIPAA accounts will be uploaded from another computer, identified by a HIPAA ID. Contact us for technical support or instructions about this subject. 

No, at the moment is software from ActiGraph incompatible with Macintosh computer systems. ActiGraph is currently developping the possibility to connect both. Of course we will keep you informed through the website about these developments.

Yet, it is possible to use ActiLife 5 or 6 and older ActiLife versions on one computer. However, new devices and older software versions cannot communicate. The following Macros and functions are usable in this case:

- Create Graphs (PDF/Exel)
- Create Caloric Output File
- Show Data Table
- Crouter 2-Regression Model
- Sleep Scoring
- Hear Rate/Energie Expenditure
- Children’s METs

Note: Download files from ActiLife 5 are no longer a *dat file format. You need to use ActiLife 5 to transform the downloaded file in the right format.

There are two editions of ActiLife; a Full Edition and a Lite Edition; Using the Full Product Key will enable all of the data analysis features of the software and can be activated on one (1) computer at a time (this number increases to 5 for Enterprise License users). If the Lite Product Key is used, only the ability to download or initialize devices will be enabled. The Lite Product Key may be used on up to 5 additional computers (15 computers for Enterprise License users). This may be used in addition to the one copy of the Full License described above. This is useful for the purposes of centrally processing the collected data using the Full Edition while the Lite Edition can be used to collect data in the field which can then be sent to the Full user for analysis. Both editions cannot exist on the same computer. 

We also have implemented portability into our licensing. It is not uncommon for a researcher to temporarily need the Full Edition if they are onsite for analysis. In order to move your installation of ActiLife from one computer to another, you must first Deactivate the license on the old computer. This will release a license and allow you to Activate it on the new PC. To do this, go to the old computer, open ActiLife, and click on Help. There is a link that says "Activation Details"; click on that and make a note of your Product Key as you will need this to reactivate the software on your other computer. Then close this window and click on Deactivate. Then click the Deactivate ActiLife button and again, follow the prompts. ActiLife will then close and you will then be able to install and activate ActiLife on a different computer. This same mechanism allows you to easily switch between the Full and Lite Editions as needed.

This procedure also applies to computers that have had Windows reinstalled or if you wish to switch between the Full and Lite version of ActiLife (and vice versa). You should deactivate ActiLife before reinstalling Windows to reduce your usage. You should then be able to reinstall ActiLife and activate it with no issues. We understand that this is not always possible (hard drive crashes, system failures, etc) but when possible, following these steps will ensure minimal interruption for users of ActiLife.

You can also use this procedure to switch between the Full and Lite editions on the same computer.

The Lite and Full version of the software are the same, the only difference is the Product Key used to activate the software. The Full Edition is required to manipulate or analyze the data collected from our devices.

Data collected by ActiSleep devices are saved on ActiGraphs server and can be exported to another computer. It is also possible to make customize reports and attach these to the patient.

Step 1. Login at http://research.artitrainer.com
Step 2. In the left corner you'll find the link to export data:


Step 3. You'll face a new screen with all users listed:

Step 4. Choose 'dat' or 'csv' to download data to your own computer

If there's bought an account, it is not possible to modify this. If there's a bigger account needed, contact us. We can change the size of your account according to your recuirements.

There are two editions of ActiLife; a Full Edition and a Lite Edition; Using the Full Product Key will enable all of the data analysis features of the software and can be activated on one (1) computer at a time (this number increases to 5 for Enterprise License users). If the Lite Product Key is used, only the ability to download or initialize devices will be enabled. The Lite Product Key may be used on up to 5 additional computers (15 computers for Enterprise License users). This may be used in addition to the one copy of the Full License described above. This is useful for the purposes of centrally processing the collected data using the Full Edition while the Lite Edition can be used to collect data in the field which can then be sent to the Full user for analysis. Both editions cannot exist on the same computer. 

We also have implemented portability into our licensing. It is not uncommon for a researcher to temporarily need the Full Edition if they are onsite for analysis. In order to move your installation of ActiLife from one computer to another, you must first Deactivate the license on the old computer. This will release a license and allow you to Activate it on the new PC. To do this, go to the old computer, open ActiLife, and click on Help. There is a link that says "Activation Details"; click on that and make a note of your Product Key as you will need this to reactivate the software on your other computer. Then close this window and click on Deactivate. Then click the Deactivate ActiLife button and again, follow the prompts. ActiLife will then close and you will then be able to install and activate ActiLife on a different computer. This same mechanism allows you to easily switch between the Full and Lite Editions as needed.

This procedure also applies to computers that have had Windows reinstalled or if you wish to switch between the Full and Lite version of ActiLife (and vice versa). You should deactivate ActiLife before reinstalling Windows to reduce your usage. You should then be able to reinstall ActiLife and activate it with no issues. We understand that this is not always possible (hard drive crashes, system failures, etc) but when possible, following these steps will ensure minimal interruption for users of ActiLife.

You can also use this procedure to switch between the Full and Lite editions on the same computer.

The Lite and Full version of the software are the same, the only difference is the Product Key used to activate the software. The Full Edition is required to manipulate or analyze the data collected from our devices.

We sometimes get inquires about determining the actual angle of inclination that our devices measure. Some researchers may need more information than just the category of inclination (i.e. sitting, standing, lying, or off). While this information is not available in ActiLife itself, you can determine this from the raw data by using some trigonometric calculations as explained below. ActiLife does not provide this information as ActiLife currently only analyzes Epoch Data.

The inclination angle(s) can be determined from the raw data. Both pitch and roll may be calculated but at this time, there is not enough data to determine yaw/heading.

Please refer to the following paper from Analog Devices that explains inclination calculations using 1-, 2- or 3-axis accelerometer data:
http://www.analog.com/static/imported-files/application_notes/AN-1057.pdf

Note that the Analog Devices paper assumes that measured acceleration is only due to gravity. So, any accelerations due to the subject must be filtered out. This can be done using a digital low-pass filter since gravity remains constant while subject accelerations change rapidly.

Tuning of a low-pass filter for gravity is dependent upon your desired results. A high-order filter with a low cutoff frequency will yield very smooth results, but will respond slowly to changes in inclination. The opposite is true as the frequency is increased. So, you have to weigh reducing noise against responsiveness. You will need to construct your own low-pass filter.

You can also refer to the Inclination whitepaper we've posted here.

Battery Life and Memory Limits vs Sample Frequency
Chart 1 assumes 16 hours of active recording time and 8 hours of idle time (low power mode).  Idle time is when the unit has not sensed activity in the past 10 seconds and goes into a low power state.  If a customer is going to do a test whereby the unit will never experience this low power mode ( i.e. continuous shaker table test or disabling the low power mode during initialization), then Chart 2 is the battery life they could expect.  The highlighted cell indicate the limiting factor in how long the unit can record data.


















Chart 1 - Battery life based of 16 hrs Active 8 hrs idle (low power mode)



















Chart 2 - Battery life based on no idle (low power mode); Unit is active for 24 hrs per day.

 Chart 3 below indicates the amount of available battery life after the device spends 10 days in delay mode (this chart also assumes 16 hours of active recording time and 8 hours of idle time (low power mode).  This typically happens when a device is deployed with a start time 10 days after the current date.  During this time, the device will remain in the idle or "low power mode" state.  The chart is used to illustrate that even after 10 days in this state, the device can still record a significant amount of data.  Note that the battery life is always the limiting factor in this case.


















 Chart 3 - Battery life vs. memory limit after a 10 day delay-before-starting.

There are a couple of things that need to be done to keep Li-Ion batteries in good condition and get the maximum life out of the battery. Units should never be left in a fully discharged state any longer than necessary as this could lead to reduced life, and could cause permanent failure. It is better for the batteries to be fully charged than it is for them to be left at a low voltage. In order to mitigate this, we recommend that units be charged immediately upon returning from the field. This will serve to minimize the amount of time the battery spends in a very low voltage state.

The recommended procedure for long-term storage of our devices is to fully charge them and then once every other month (once every three months at the most), connect them to a computer or USB charging hub for 3 or 4 hours and allow them to fully recharge before once again storing them in a cool, dry place.

Storing the batteries in a refrigerator (or some other cold storage) really will not add much in terms on prolonging battery life. When the devices are in low power mode, they consume approximately 86µA (micro-amps) of current, which will give you about 80-90 days of storage before the device needs to be recharged. Storing them in a refrigerator might reduce that power consumption by 5µA to 10µA, which really only gets you about another 7 to 15 days of storage before the batteries are completely discharged. And actually, refrigeration can be detrimental because, as you probably know, there is added moisture in a standard refrigerator and if moisture gets inside of the devices and shorts out the electronics, the devices will need to be discarded. Allowing the batteries to completely discharge can also render them useless.

Leaving the devices connected to a charging source all the time will not damage them. The batteries do have a "shut off" mechanism that will keep them from overcharging, but it is not recommended for environmental reasons as this is a waste of electricity.

This chart shows impact of battery charge and ambient temperature on battery capacity during storage.

After 300 charge/discharge cycles (charge to 4.2V then discharge to 3.0V) the capacity can drop to as low as 80%

Lux, or ambient light, is measured by ActiGraph’s ActiSleep, ActiSleep+, ActiTrainer, GT3X+, and our new wireless wGT3X+ and wActiSleep+ devices. Ambient light may affect subject sleeping habits and thus is a useful tool in analyzing circadian rhythms and sleeping patterns. Lux data is stored once per epoch. For GT3X+/ActiSleep+/wGT3X+/wActiSleep+ devices, Lux data is stored once per second. When converting a GT3X+ raw file into an accumulated *.agd format with epoch lengths greater than one second, the lux values for that epoch are averaged. An estimate of industry accepted lux values is shown below. The ActiTrainer, GT3X+, and wGT3X+ devices are capped at a maximum value of 2500; the ActiSleep, ActiSleep+, and wActiSleep+ are capped at 6000:

Note that these levels are only estimates and are not meant for exact interpretation of the light detected by the device. Lux values reported by ActiGraph devices are intended as a general guideline for average light intensities.

We will begin with the very first thing you see when you open ActiLife; the Devices Tab. This is where you will perform all interactions with your Devices; Initialization, Downloading, Firmware Updates, etc. You will notice that there are a number of columns that provide information about the device itself; the Serial Number, the Battery Level, Sample Rate of the data being collected, etc. There are also several buttons along the top of the Grid that allow you to perform actions on the devices that are connected.

• The Initialize button is used to prepare the device for data collection.
• The Download button is how you retrieve the data from the device and get it onto your computer for analysis.
• The Refresh and Refresh All buttons allow you to reload the device grid to make sure all of the devices are visible and reporting the correct information. it's the same idea as refreshing a webpage; you are just reloading information that is being reported by the connected devices to ActiLife. Refresh only refreshes selected devices; Refresh All refreshes the entire device grid.
• The Identify button allows you to cause a device's LED to flash so that you know which device you are about to manipulate. This is useful when you have multiple devices connected but perhaps only wish to download or initialize a single device. Using the Identify function will help you make sure you are performing whatever actions you want to on the correct device.

For the purposes of this tutorial, we will not go into detail explanations of the Wireless Functionality. That will be covered in another tutorial that will be posted at a later time.

 

The next step after retrieving your data is to begin analyzing it. The first step in this process is to validate the data for Wear and Non Wear-time. Wear-Time Validation (also known as WTV) is the method by which ActiLife flags Epoch data as Wear-time (i.e. yes, the device was being worn and the subject was doing stuff) or Non-Wear time (i.e. no, the device was not being worn or the subject was not doing anything). This is achieved by applying an internally developed algorithm along with other customizable criteria to the dataset. We will not go into detail about what each of these criteria are here but you can find out more about them by viewing the FAQ articles posted here.

On the left side of this tab, you will see the various criteria that you can adjust for your specific study (in most instances the defaults are fine). The buttons along the top of this tab allow you to add or remove Dataset(s). Simply clicking on the Calculate button will analyze the dataset using the selected criteria. You can use the scroll bar at the bottom of the window to see the results for the entire dataset. If you would like to view the results down to the Epoch level, click on the details button next to the dataset.

This takes us to the next tool in ActiLife; the Data Scoring tab. 

Once you have validated your data, the next step is to actually analyze it. ActiLife's Data Scoring tool has the ability to analyze a dataset for all of the primary criteria relevant to actigraphy; Energy Expenditure, METs, Cut Points, Bouts, Heart Rate, and Sedentary Analysis. You can read more about these various criteria here.

Along the left side of the Data Scoring Tab, you will see the various criteria you can use. You can select different algorithms from the pull down menus and in the case of Cut Points, Bouts, and Sedentary Analysis, even create your own. You can also add Data/Time filters that will allow you to specify that only certain days or time periods be included in the analysis.

The buttons along the top of the window will allow you to add/remove datasets and to customize which columns are displayed. The number of currently loaded datasets is also display in this area. The buttons along the bottom allow you to perform the analysis and to export the results out to either a CSV or Microsoft's Excel XLSX file format.

 

Once the data has been scored, you can click on the details button (next to the dataset filename) to see a breakdown of the data down to the Epoch level. If the data was scored for Sedentary Analysis and/or bouts, you will also be able to see a breakdown of those as well.



To add your own custom Cut Point sets, simply click on the edit button next to Cut Points to open the Cut Point Editor. You cannot edit the existing Cut Points but you can add a new one based on an existing one and then adjust it to suit your requirements.



You would do the same thing to create your own Bout definitions.




















You can also adjust the options for Sedentary Analysis.



















For many users, this is as far as they might go in analyzing their data. However, there are several other analysis tools in ActiLife for users with other requirements such as Sleep Analysis and PLM. For an explanation of the Sleep Analysis Tab, click here.

The next tab we will explore is the Sleep Analysis Tab. Unlike the WTV and Data Scoring Tabs, you can only use this tool with one dataset at a time. This is simply due to how this data is displayed using graphs as opposed to rows and columns of data. There is simply no practical way to display all of this information for multiple files on the same screen.

There are a number of functions here to make note of: the Select Dataset button allows you to load your data into the Sleep Analysis tool. You also have the option of select either a 24 or 48 hour scale for the graph. The AutoScore feature will attempt to automatically detect Sleep Periods. You can also switch between the Cole-Kripke and Sadeh sleep algorithms. More information on these algorithms can be found here. Using ActiSleep/ActiSleep+ devices also allows the ability to create custom sleep algorithms.

Once you have either Auto-scored your sleep periods or manually entered them, you will have the option to export the scored data as a PDF or as a CSV file. Please note below that after I have entered my sleep times, there is information about those sleep time listed in the lower right pane of the Sleep Analysis tool.























You can also switch the view to the ActoGram View which is helpful identifying sleep patterns over several days or weeks.

Whichever view you use, you can see more detailed information about the scored sleep periods by clicking on the "Show Sleep Epochs" button. YOu can also export using the Save Sleep Report button.

Please note; the Auto Sleep Score, Custom Sleep Algorithm, ActoGram View, and Clinical Reports functions in ActiLife are only available for our ActiSleep/ActiSleep+ devices. However, these features can be enabled for other devices for a small fee. Please contact our Sales team at sales@actigraphy.nl for more information.

Next we will go over the Periodic Limb Movement (or PLM) Analysis tool. 

One of the most common questions we often get from our customers is "How do I use my devices and ActiLife?" and "I really have no idea where to even start. Can you please help me?". The purpose of this article is to hopefully provide a step-by-step guide to get the most from our state of the art Activity Monitors and the powerful suite of Data Scoring and Analysis tools found in ActiLife. For the purpose of this tutorial, I will be using ActiLife 6.4.2 installed on 64-bit Windows 7 Professional and one of our new ANT+ Enabled wireless devices, the wActiSleep+. While some of the options available to you may differ depending on the device you are using and the version of ActiLife you have installed, it should all be very similar.

If you have not yet installed ActiLife, please do so by following the steps outlined in this FAQ article.

The very first thing you need to do is make sure your device is charged. All devices ship from us in a partially charged state and in "Halt" mode. This simply means that the device is in a low power state and needs to be initialized before being deployed (think of it as similar to needing to fully charge and activate a new cell phone before you can use it). Charging our devices is a very simple process; simply connect the device to an available USB port on your computer using a USB cable. If there are no problems with the device, the USB port or the cable itself, the LED on the device should begin to blink, indicating that it is charging. Please refer to this FAQ for more information on what the various LED patterns mean. Fully charging a device can take 2 to 3 hours on average, depending on the level of charge on the battery. Once the device is fully charged, the LED will remain solid while connected to your computer.

Once ActiLife has been installed and activated and the device has fully charged, we're ready to get started. There are five basic steps involved in this process; Initialization, data collection, downloading that data, validating wear-time, and analyzing the data that you have collected. We refer to these 5 basic steps as the ActiLife WorkFlow Process.

• Initialization refers to the process of preparing the accelerometer to collect activity data from your research subjects.
• Data Collection refers to the act of collecting activity data from your subjects.
• Downloading means retrieving the collected data from the device and getting it onto your computer for analysis.
• Validating Wear-time refers to the process of flagging activity data as either Wear-Time (the subject was wearing the device and engaging in some sort of activity) or Non-Wear Time (the subject was either motionless or not wearing the device) using internally developed algorithms.
• Analyzing refers to scoring the data using the tools in ActiLife (Energy Expenditure, METS, Sleep analysis, etc).

We will go into details on all of these steps later in this FAQ. While you may not be responsible for all of these steps in your research (i.e. you may only be downloading devices or scoring data collected from other researchers), understanding the work flow is beneficial because you will understand how each step is an important part of the process.

At the end of this overview of ActiLife, we will take a step by step walk through of the actual workflow process from start to finish using Epoch data created from sample activity data so that you will have a solid understanding of this Work Flow Process.

First, it might be helpful to go over the various tabs in ActiLife and briefly explain how you will use the different areas regardless of whether you are doing everything yourself or if you are only responsible for one particular aspect. 

All ActiGraph devices are manufactured in a way that preventive maintenance (calibration) isn't necessary. To be sure that the devices function properly and data are stored correctly, we recommend visual inspection of the devices. This is especially true for devices which are waterproof; the o-valve/cartridge should be checked to be sure there are no cracks.

The devces could be cleaned with an isopropyl alcoholic piece of cloth. 

Note: the user cannot, without any exceptions, strip down the device for inspection. Warranty will expire! 

This article addresses the process in which ActiGraph devices (GT3X+ and later) capture raw data and convert it to epoch data. Information beyond what is provided in this paper will not be disclosed to non ActiGraph employees as it has been deemed proprietary/company confidential. As such, release of this information could prove detrimental to ActiGraph’s business interests.

Beginning with the release of the ActiGraph GT3X+, the company shifted its data collection/storage focus from solely filtered/epoch-based data to raw accelerometer data. Prior to this shift, ActiGraph devices performed all data filtering and processing within the hardware, resulting in data being delivered to end users in epoch accumulated format.

The ActiGraph GT3X+ records accelerations ranging in magnitude from +/- 6 g’s. The accelerometer output is sampled by a twelve-bit (12) Analog to Digital Converter according to end user settings, which can range from 30 Hz to 100 Hz. This raw acceleration is then stored in non-volatile flash memory for future analysis.

Utilizing the ActiLife software application, the raw data collected by the GT3X+ can be reviewed directly or further processed through ActiGraph’s digital filter. This digital filter band-limits the accelerometer to the frequency range of 0.25 to 2.5 Hz, which has been carefully chosen to detect normal human motion and to reject changing accelerations outside the pass band. Each sample is then summed over a user specified interval of time called an ‘epoch’

Figure 1 depicts this process.















 

When exporting raw *.gt3x files to *.csv (by using the Raw to Raw function from the Import/Export Menu), users have the option to "Include Timestamps".  This option places a new column in the resultant *.csv output that includes the full date and time stamp, to the millisecond.

You can also set this as the default behavior by going to Tools, then Options, and clicking on the Downloads Tab:

A "dot" notation is used to indicate the millisecond timestamp for each record in the raw data.  Below is an example portion of a raw data export recorded at 30Hz.

Note that the addition of the timestamp can more than double the overall size of the *.csv export.
Date formatting will be specific to the local exporting computer's culture settings.  The time portion, however, will always be a 24-hour (to the millisecond) representation.

No, the ActiGo is usable separately from the ActiTrainer. For more information go to http://www.actitrainer.com/

The defaults for Floating Window Wear Time Validation were not derived from any specific source.  However, the NHANES SAS code and comments (available at http://riskfactor.cancer.gov/tools/nhanes_pam/) from the 2003-2004 NHANES data set were used to derive the adjustable parameters.  While the 60s consecutive "zeros" and 2 minute "Spike Tolerance" defaults do match this documentation, by default the "Spike level to stop" option is disabled.  In order to match the NHANES SAS code, this option should be enabled and set to 100.  If enabled, it is defaulted to 100.

The lates release you can always find on http://www.actigraphy.nl/en/downloads
 

Note: software always need to be activated before you can fully use it. Contact us when there are questions left.

http://www.actigraphy.nl/nl/downloads

1. Go to http://www.actigraphy.nl/en/downloads
2. choose for the right product
3. download the manual 

1. Go to http://www.actigraphy.nl/en/downloads
2. choose for the right product
3. Below you'll find the manual for your product

http://www.theactigraph.com/article/research-database/a-refined-2-regression-model-for-the-actigraph-accelerometer/

There are several ways to find the serial number of your device.

The latest devices will have a sticker with the serial number. If this is not the case, you can go through the steps below to find the serial number:

In ActiLife:

In ActiSync:
Look for the ActiSync pictogram in the right corner below on the desktop and click on it with your right mouse button.


In ActiSleep\ActiTrainer (legacy) Software 
 

When you have an ActiTrainer, you need to take the steps below to find your serial number and firmware version:

1. Click on 'select' to open the ActiTrainer menu
2. Scroll to ‘DEV INFO’ and select
3. Then select ‘REV INFO’
4. The firmware version and serial number will be shown

Below the prefixes of the different serial numbers from the different devices are shown:

Device Prefix
GT1M  LYN
GT3X  MAT
GT3X+ NEO
ActiTrainer PUL
ActiSleep MID
ActiGo  MOR

All ActiGraph activity monitors are designed to monitor human activity and record energy expenditure (caloriesspent during normal activity, METs, everyday activity, and exercise). Additionally, these devices can alsofunction as a very accurate sleep assessment tool. While collecting day-to-day energy expenditure data, thedevice should be affixed securely to the body’s center-of-mass to ensure the most accurate caloricmeasurements. Wrist worn (nonvalidated) EE for kids is now available for 3-axis actigraphs. When being usedfor sleep assessment, the ActiGraph device may be worn anywhere on the body: wrist, waist (hip), arm, orankle.

Note: It is not safe to wear the ActiGraph while it is charging!

To use the elastic waist belt, lace the beltthrough the side loops of the actigraph device and secure the belt around the waist. The unit can be worn eitherabove or beneath clothing, and it is not necessary for the device to make contact with the skin. However, thedevice must be held snugly against the body to prevent erroneous readings. To use the belt clip, attach the clip tothe back of the actigraph using the double-sided adhesive strip and attach the unit securely to a belt on thesubject’s hip.

Note: The belt clip adheres permanently to the ActiGraph. Once the belt clip is attached to theback of the ActiGraph, you will not be able to wear it on the elast belt comfortably.

It is important to determinewhich method is best for you before the belt clip is attached to the back of the ActiGraph. It is recommendedthat children under 12 years of age wear the Actigraphs on the elastic belt (versus the adhesive belt clip) as it isthe most secure way to wear the device. Belt loop pouches, wrist and ankle straps are also available.

The samerecommendations apply to the ActiTrainer: the device should be worn on the waist using the supplied neoprenepouch and belt for normal, daily activity but can be worn on the wrist, ankle, or upper arm for sleep assessment.The ActiTrainer may be worn on the arm using a neoprene armband; however, energy expenditure calculationswill not be as accurate at this location.

The ActiTrainers manufactured after July 1, 2009 have a 3 axisaccelerometer as do the GT3X series and ASM Actigraphs. The ActiTrainer memory has also been expandedto 4 MB, four times storage capacity of the earlier model.

The GT3Xs, ActiTrainers with 3 axis accelerometersand the ASM have an Inclinometer feature. The Inclinometer will only function properly when the device is wornat the hip area of the waist.

• Kicks - Kicks are simply movement of the foot indicative of typical peridoic leg movements detected by PSG.  The device is worn on the dorsum of each foot at the base of the big toe and hence is more sensitive to "kicks" as might be detected by electromyograph (EMG) with electrodes placed on the tibialis anterior muscles.  The decision to place ActiSleep devices on the to area was based on the research performed by Kemlink, et., al. in A comparison of polysomnographic and actigraphic evaluation of periodic limb movements in sleep.  The concept of kicks and their relation to EMG were derived from M. King, et.al in The validation of a new actigraphy system for the measurement of periodic leg movements in sleep and from E. Sforza, et.al. in The PAM-RL ambulatory device for detection of periodic leg movements: a validation study.  Click on the paper to read the abstracts.  
• PLMS - Periodic Leg Movement during Sleep.   The definition of periodic leg movement used in ActiLife is the same as the definition found in the research paper by Gschliesser, et.al., PLM detection by actigraphy compared to polysomnography: A validation and comparison of two actigraphs.  Accordingly, PLM are defined as "a sequence of four or more leg movements separated by at least 5 (and not more than 90) seconds with a duration between 0.5 and 5 seconds."  Since the minimum epoch allowed in ActiLife is 1s, our criteria for defining a PLM is a duration between 1 and 5 seconds.  A minimum of four "kicks" (or, leg movements) are required to start a PLM bout (or PLMS).
• PLMS Bouts - PLMS bouts are simply the consecutive bout time representing unbroken PLM.  A PLM is disqualfiied when the separation between kicks exceeds 90 seconds or does not reach 5 seconds.  This information was also gathered from the research paper by Gschliesser, et.al.,http://www.actigraphcorp.com/research-database/plm-detection-by-actigraphy-compared-to-polysomnography-a-validation-and-comparison-of-two-actigraphs/

There is a possibility that you don't have to pay the full price for ActiLife 6. Contact us for more information: support@actigraphy.nl

HREE stands for "Heart Rate Energy Expenditure."  This feature works with *.agd files that contain heart rate data (beats per minute) collected from a wireless heart rate transmitter.  Compatible ActiGraph devices include the ActiTrainer (obsolete), the wGT3X+ and the wActiSleep+ monitors.  "w" devices collect heart rate data over a wireless ANT+ channel from the ANT Heart Rate Monitor sold through the ActiGraph online store.

When an *.agd file contains heart rate information, the HREE feature uses that heart rate information to estimate energy expenditure during times of low ambulatory movement.  Basically, this algorithm examines the subject's heart rate during high activity and during low activity (to obtain a baseline) to determine an equation that can be used whenever activity levels are low and heart rate levels are high.  This equation can be used by the researcher to estimate calorie burn during non-ambulatory times (e.g., when someone is lifting weights or cycling).  

Note: This method is not validated by any independent research organization.  It was derived by ActiGraph's R&D department and has not been studied on large populations.

The HREE tool calculates 5 variables
ADL Heart Rate - This is the Average Daily Living Heart Rate for the data set.  Ever 60s epoch with a count level greater than 100 counts per minute (CPM) and a heart rate between 41-79 beats per minute (bpm) gets averaged.  This average represents the Average Daily Living Heart Rate
Avg Active Heart Rate - The average heart rate for all 60s epochs with greater than 1951 CPM.  The heart rate value for the epoch must exceed 79 bpm in order to be counted as part of the average
Heart Rate Delta - The difference between the Average Active Heart Rate and the ADL Heart Rate.
Avg Active Caloric Expenditure - The average calorie burn (in kcals) for all epochs with a heart rate value >79 bpm and count levels >1951 cpm
Calibration Ratio - The slope of the heart rate - energy expenditure line (Avg Active Caloric Expenditure-Average Baseline Calories)/(Avg Active Heart Rate-ADL Heart Rate)

 

Note: The Average Baseline Calories value is not calculated as part of the HREE output but is just an intermediate variable.  This value represents the average calorie burn (in kcals) for all epochs with a heart rate value between 41-79 bpm and count levels >100 cpm

In order for the HREE tool, the *.agd data set must contain at least one epoch where axis1 > 100 cpm AND HR is >= 41bpm and <= 79bpm AND at least one epoch where axis1 > 1951 AND HR >= 80.

All possible pictograms are shown below. Not all pictograms are available in all devices:

The "Worn on Wrist" option allows users to tell ActiLife that the device was worn on the wrist and that the calculations in Data Scoring should be scaled appropriately.  At the time of this writing, there are no validation studies available that illustrate how to properly scale wrist worn devices to yield kCal information. Waist-worn kCal calculations are still the defacto standard and research studies have proven the reliability. That said, ActiGraph applies the following piece-wise scaling to wrist-worn devices based on internal research and development:

Wrist count Equivalent Counts

0
0 - 644 0.5341614 * wrist count
645 - 1272 1.7133758 * wrist count - 759.414013
1273 - 3806 0.3997632 * wrist count + 911.501184
3807 - infinity 0.0128995 * wrist count +  2383.904505

The resultant counts are used to calculate kCals and Cut Points in the Data Scoring tool. 

Sleep efficiency or 'sleep efficiency index' is the amount of sleep in the nightly period when the person was really asleep, so the ratio between the total sleeping time and total time in bed. Normally this ratio should be at least 85%. This value can be lowered by for example insomnia (source: American Sleep Apnea Association).

This function removes all information from patients which is saved on ActiGraphs server and on other places with the help of a unique HIPAA-ID. The names of the patients will be saved on the user pc and will stay visible on the pc the device was uploaded.

ANT is an efficient low power, wireless protocol that operates in the 2.4GHz ISM RF band.  ActiGraph's wGT3X+ accelerometers contain hardware that allow them to communicate with ActiLife, ActiLife Mobile, and other peripheral devices using the ANT protocol.  Because of bandwidth limitations, ANT wireless communication with ActiLife is limited to status-type information including battery life; remaining memory; and daily and overall wear time, kcal, and cut point summaries.

More information about the ANT protocol can be found at http://www.thisisant.com/ and http://en.wikipedia.org/wiki/ANT_(network)

API Overview
The ActiLife API or "Application Programming Interface" is a new feature available in ActiLife which allows programmers to access key ActiLife functionality from within their own custom applications.  Currently, the API provides control of USB device initialization and download, wireless initialization, wireless burst downloads, LED identification, and ActiLife application launching, minimizing and restoring.  More features are being added daily.  

API Use Cases
The API feature is designed for our customers who require specific and consistent responses from our ActiLife software through their own custom web or client-side applications.  Examples include:

• Pharmaceutical clinical trials in which ActiLife is required to operate within a 21 CFR Part 11 compliant system
• Large scale research projects that require consistent data capture from lab technicians who are geographically dispersed
• Web applications designed to capture objective actigraphy data from patients (wired or wirelessly)

Enabling the API Feature
The API feature is currently enabled manually by an ActiGraph customer support representative.  Contact ActiGraph for more information, including pricing, at sales@actigraphy.nl

Documentation
The ActiLife API documentation is updated continuously as the API features evolve.  The live document can be viewed at http://www.actigraphcorp.com/support/API

Priorities for Additional Functionality
The ActiLife team is currently actively working on the following improvements to the API:

• Offline activation - ActiLife currently requires an active Internet connection to function.  This requirement is being removed in version 6.7 and up
• Additional Data Processing - The API currently does not support Wear Time Validation or Data Scoring functions.  
• File Formatting on Download - Currently, file formats cannot be selected (only *.agd and *.gt3x files are supported)
• Firmware and Application Auto-update Control - Currently, firmware and application updates occur automatically.  Many of our API customers need the ability to control this in the field.
• Addition of a Factory Reset for Devices - The API will allow users to restore devices to factory settings for long storage periods.

Our Low Frequency Extension (LFE) filter is designed to detect lower amplitude movements over our standard filter. The sensitives of solid state MEMS accelerometers like the one utilized in ActiGraph's products are very high and can detect even the most minute of movements (+/- 6G's for our GT3X+/wGT3X+/ActiSleep+/wActiSleep+ devices and +/-3G's for our legacy products).

Because we are only interested in movements that are attributable to human activity and not minor vibrations found in our everyday environment, we have an internal acceleration threshold that must be crossed before we consider it human movement related and as a result, record it. This threshold limits the ability of the device to measure movement of populations such as the elderly or any population that is very slow and/or exhibits very low acceleration outputs.

In order to combat this, and in order to provide researchers the ability to gather useful information about this subject group we have provided the LFE which reduces this threshold. The net effect is that the reduced amplitude movements are now recognized and recorded, yielding information of value.

This modus mentioned if the device has too few battery (<3.1V) to be able to collect data. This modus ensures that acceleromter data is being saved in the flash memory of the device, before the 3.0V is reached and the device fails.

 Because the device's internal clock stops and the 'HALT' modus went on, the device cannot be simply charged and collect data again. The device must be connected to the computer, the information should be downloaded and the device needs to be initialized again. In the ActiTrainer device, the 'HALT' symbol is shown on the display. The LED of the other devices will blink 3 times before the HALT modus starts.

Older versions of ActiLife produced *.dat files when downloading data from devices. These files consist of ten(10) lines of header data (meta information about the content) followed by epoch accumulated/filtered data. This“DAT” file stores all of the activity (1 to 3 axis), pedometer, Inclinometer, and/or heart rate data in ASCIIformat. This file also contains the Serial Number, Start Time, Start Date, Epoch Period, Download Time,Download Date, Current Memory Address Pointer, Current Battery Voltage, Mode and First Start Time (usedto build the DAT file) as part of its ten (10) line header information. The file can be viewed with any standard texteditor such as MS Notepad. The Mode value given in the header of the DAT file outlines which features wereactive during data collection for the file. 



The "DAT" file data isinterleaved in the following priority order based on the Mode value. Device .DAT file data priority (order ofappearance):
GT1M/GT3X/ASM, Activity, Steps
ActiTrainer, Activity, Steps, Heart Rate, Workout Activity (0unless device is in workout mode), Workout Steps (0 unless device is in workout mode)

Note that theActiTrainer records workout data in a separate memory slot. This separation is done primarily for commercialcustomers, runners for example, that are interested in obtaining the exact number of steps taken or distancetravelled as soon as a workout is enabled, which could occur in the middle of an epoch. Workouts can only beenabled while the ActiTrainer display is enabled and only during 60 second epochs.

The data table in the *agd database consist of the following parameters: 

TimeStamp – a measure of total Ticks. A single tick represents one hundred nanoseconds or one tenmillionthof a second. There are 10,000 ticks in a millisecond. The value of this property represents the numberof 100-nanosecond intervals that have elapsed since 12:00:00 midnight, January 1, 0001. More informationavailable at Microsoft (http://msdn.microsoft.com/en- us/library/system.datetime.ticks.aspx)

axis 1 – Post filtered and accumulated per-epoch vertical activity data

axis 2 (optional)– Post filtered and accumulated per-epoch horizontal activity data

axis 3 (optional) – Post filtered andaccumulated per-epoch perpendicular activity data

steps (optional) – Total steps per epoch

hr (optional) –Heart beats-per-epoch. This data is only gathered by ActiTrainer devices when those devices are initialized tocollect heart rate data AND when the Polar® Heart Monitor (wireless heart strap) is worn by the user.

Lux(optional) – Ambient light sampled once per second (on GT3X+ and ASM devices only) and averaged over thelength of the selected epoch. (e.g., for 60s epochs, this would be the light values summed once per second anddivided by 60).

Inclinometer (optional) – Device orientation information. This is calculated by sampling theangle of the device over the entire length of the epoch (30 times per second) and selecting the predominant angle;i.e, the angle “range” that appeared the majority of the time over the epoch.

The Sadeh algorithm was derived from fundamental research performed by Avi Sadeh, Katherine Sharkey, and Mary Carskadon entitled Activity Based Sleep-Wake Identification: An Empirical Test of Methodological Issues.  This paper is available on ActiGraph's research database here.  This algorithm is primarily used for younger adolescents as most of the research was performed on children and young adults.

Williams Work-Energy ('98).  This formula utilizes the physics equivalent of energy and was derived by CSA (ActiGraph) engineer Robert Williams in the paper Kcal Estimates from Activity Counts using the Potential Energy Method.

kcals=CPM×0.0000191×BM

where

 kcals=Total Calories for a Single Epoch
CPM = Counts per Minute
BM = Body Mass in Kg

The linearity of this equation allows ActiLife to scale non-60 second epochs up to their 60s equivalent then back down again to obtain a per-epoch kcal value. 


Freedson ('98).  Developed by Dr. Patty Freedson in 1997 in the research publcation Calibration of the Computer Science and Applications, Inc. Accelerometer, this equation is only accurate above 1951 counts for adults. 

 

All ActiGraph devices have a low-power modus (LPM) where the device the power automatically lowers during periods of sedentair acivity. LMP will be active then 60 seconds no activity is recorded. The power of the device will significantly be lowerd which can extend the lifespan of the battery upto 15%.** When the device is in LPM mode, every second the activity will be checked on the X and Y axis. When activity is detected, LMP modus will be immediately deactivated and the device measures as usual. Since July 2012 it is also possible to deactivate this function.

** The lifespan is changing continuously because ActiGraph is always working to lenghten the lifespan of the batteries.

The *.agd file format is ActiLife’s native file format and is the desired file type for all ActiLife operations andtools such as Wear Time Validation, Data Scoring, Sleep Scoring, and Graphing. *.agd files are database filesformatted for use with the popular SQLite architecture (www.sqlite.org). These files can be viewed in theActiLife AGD viewer (available from the File menu). Alternatively, the SQLite browser (publically available onthe SQLite website) can be used to browse the details of the *.agd files. AGD File Schema: *.agd files arecreated in ActiLife immediately when data from a GT1M, GT3X, ActiTrainer, or ASM device is downloadedthrough the “Devices” tab. These *.agd files typically contain post-filtered and accumulated data as this is theonly type of data that ActiLife can properly handle. The rows in the database represent whole-number epochsummations (1s, 5s, 10s, 30s, 60s, etc.). However, at the time of this writing all GT1M, GT3X, ActiTrainer, andASM products that have been initialized to collect raw data (12Hz or 30Hz) will produce an *.agd file ondownload. Although the file cannot be processed in ActiLife, the *.agd format can be exported to *.csv or *.datthrough ActiLife’s import/export tool or AGD viewer tool, both accessible from the File menu. GT3X+ devicesproduct a *.gt3x file on download (see *.gt3x File Format). In order to process in ActiLife, the file must beexported to *.agd format. This can be done at the time of download by checking "Create AGD File" from thedownload dialog box or by using ActiLife's built-in import/export tool available from the File menu.

The Floating Window Criteria settings are used to define windows of inactivity. These windows of inactivity(also called "non-wear periods") can be flagged and excluded from further analysis within ActiLife. This meansthat you will have the option in the Data Scoring tool to exclude these "non-wear periods" from your analysis.This is useful if you're trying to analyze periods of real activity rather than periods when the subject did not wearthe device. Wear Time Validation is used to:

1. Exclude non-wear activity periods from further data analysis (asexplained above) 
2. Simply identify periods when the subject did not meet prescribed activity requirements(i.e., the user was inactive for large amounts of time while wearing the device).

The Floating Window Criteriaparameters are explained below.

- Valid Activity Floor - The minimum count level per minute to qualify a "valid"activity period. Note that this value is scaled to one minute intervals. For example, if the Valid Activity Floor isset to 240 counts per minute, this implies a floor of 40 counts per epoch for a 10 second epoch file. In thisexample, each epoch that exceeds 40 activity counts will contribute 10 seconds of wear-time" toward the weartimesum. Once the sum of the wear-time" exceeds the "Flag After..." length (in minutes), the non-wear periodwill end and a wear-period will begin.

- Use Vector Magnitude - When checked, the Valid Activity Floor appliesto the vector magnitude of the 3-axis data collected by the device. If the device does not have 3-axis dataavailable, only the vertical axis is used. When unchecked, only the vertical axis data is used to calculate weartime validation.

- Flag as "non-wear" after.... - The minimum total of non-wear time required to score a period asa non-wear period. In the previous example (using 10 second epochs), a minimum of 60 consecutive epochs(assuming the default "Flag as "non-wear" after..." time is 10 minutes) must be encountered in order to score aperiod as "non-wear" time. This example does not take into account the Drop Time or tolerance which isexplained below.

- Drop Time (tolerance) - The drop time provides the user with a tolerance window for nonwearperiods and allows the user to take into account (i.e., filter out) small spikes in activity which should not beconsidered valid wear time. In the previous example, setting the drop time to a default of 2 minutes means that anon-wear period may contain up to 12 epochs (not necessarily consecutive) of data greater than 40 counts. Anon-wear period will no longer be scored as a non-wear period when the tolerance is exceeded.

• Minimum Length (minutes or epochs) - This option sets the minimum required number or consecutive zeros (or data below the Activity Threshold if that feature is enabled) that must be encountered before a period is considered a "non-wear period."  After scanning the data and detecting the selected number of consecutive zeros, ActiLife will begin flagging data as "non-wear time."  The leading zeros detected during this process will be flagged retroactively (after they have been initially scanned).  Data will be flagged as "non-wear time" until the Spike Tolerance is exceeded.  That is, until the non-wear bout is broken by activity that exceeds 0 (or the Activity Threshold) for the number of times defined by Spike Tolerance.
• Use Vector Magnitude - When checked, ActiLife scans the vector magnitude of three-axis data (when available) to detect wear or non-wear time.  When unchecked, ActiLife scans only Axis 1 (the vertical axis - see users' manual for details) to detect wear/non-wear times.  
• Activity Threshold (per minute or per epoch) - This value represents the non-wear "floor."  When this feature is disabled, a non-wear bout is identified as periods of zeros (0s) in the data.  When enabled, any epoch below the set value will be considered as "zero."  Any epoch level over this threshold is considered a Spike
• Max count level (per epoch or per minute) - Setting this option will flag epoch (or minute) counts that exceed this threshold as "zeros".  This option is useful if the dataset contains some corrupt data, for example, with large count levels.  This option can be used to exclude those count levels from wear-time analysis.
• Spike Tolerance (number of minutes or epochs) - ActiLife will continue scoring a non-wear bout as non-wear until it detects more than the Spike Tolerance number of epochs above zero (or above theActivity Threshold, if that option is enabled).
• Spike level to stop (count level per epoch or per minute) - If this option is set, a non-wear bout will end if a count level that exceeds this value is encountered.  This option allows the Wear Time Validation tool to mimic exactly the behavior of the 2003-2004 NHANES SAS code which eliminates non-wear time using the same technique.

• Ignore wear periods less than _____ (minutes or epochs) - Because of the WTV algorithms used, some wear periods may be very short (shorter than the spike tolerance).  Use this option to set the minimum length of an acceptable wear period.
• Minimum wear time per day (minutes or epochs) - Use this setting to flag an entire 24 hour day as invalid if there are less than the selected amount of valid (wear-time) minutes or epochs in the day.  
• Minimum days of valid wear time - Use this option to flag an entire dataset as invalid if there are less than the minimum number of valid days.

The minimum System Requirements for ActiLife 5.x/6.x are as follows:

• Microsoft Windows XP (32 bit only), Vista/7/8 (32 and 64 bit)
• 1 GHZ 32-bit (x86) or 64-bit (x64) processor
• 1 GB of system memory
• 500 MB of available hard drive space
• USB 1.1 Compliant Port (2.0 recommended)
• Internet Access (for auto-update and Data Vault features)
• Microsoft Dot NET Framework, 3.5, SP1 (Web Installer here; Full installer here.)

The Wear Time Validation tool in ActiLife allows users to screen epoch-level *.agd files (collected from any ActiGraph device) to flag periods of non-wear.  Non-wear is estimated by analyzing periods of little or no activity and applying algorithms to those periods to determine if the user was actually wearing the device or not.  

Although ActiLife offers two Wear Time Validation algorithms, the "Daily" algorithm is deprecated - users are advised not to use this algorithm.

This article aims to describe the difference between the "Daily" and the "Floating Window" wear time validation algorithm.

Commonalities
Both algorithms have the following features in common.

• Detects wear-time vs. non-wear time by examining the number and behavior of "zeros" in the data
• Flags, does not modify, epochs within the *.agd file as "wear" or "non-wear"
• Gives the ability to characterize a "zero" as being higher than "zero" (activity floor) or above a certain high threshold (activity ceiling)
• Flags an entire day within a dataset as invalid if minimum "valid hours" requirement are not met
• Flags an entire dataset as invalid if minimum "valid days" criteria are not met

"Daily" Wear Time Validation

The "Daily" option breaks a file down into calendar hours and calendar days.  Essentially, an entire HOUR will be flagged as valid or invalid based on the validation criteria.  At the end of each hour, the analysis starts over.  

"Floating Window" Wear Time Validation

The "Floating Window" algorithm does not break a file into hours but rather looks at consecutive epochs for patterns.  Users can choose, for instance, to say "only consider 75 minutes of consecutive zeros as non-wear".  After that pattern is detected, all data within that range and the data that follow will be flagged as non-wear.  There are no calendar hour/day constraints to detecting non-wear time.  This algorithm provides the user with much more flexibility by allowing the user to set tolerance levels, minimum wear-time lengths, and non-wear stop levels.  For a full description of the options available in this algorithm, see Wear Time Validation Parameters.

GT3X+ devices produce an interim compressed file with file extension *.gt3x. This is a compressed file and mustbe extracted to a usable format using ActiLife’s import/export tool which is accessible from the File menu inActiLife. The file can be exported to *.agd format for processing in ActiLife or *.csv/*.dat format for processingusing third party tools. The *.gt3x file can also be exported directly to *.agd format during the download processby checking “Create AGD File” from the download prompt.

ActiLife offers 12 different MET algorithms to determine the average hourly and daily and per-epoch metabolic rate (link to Wikipedia article on Metabolic Rate) for datasets.  In short, 1 MET represents the amount of energy the human body expends at rest.  This is equivalent to their Basal Metabolic Rate (or BMR).  A MET rate of 2.0, for instance, indicates that during that time period, the subject was expending twice their normal sedentary energy (BMR*2).  This value will never fall below 1.0.

All of the MET formulas used in ActiLife and listed below identify the MET rate using Counts Per Minute (or CPMs - see "What are counts?" for more info).  Files that contain sub-minute data (e.g., 10s epoch data) are scaled up (multiplied by a factor to obtain the 60 second equivalent).

Freedson Adult (1998) -

Freedson Treadmill Adult (1998)

• Formula: MET Rate = 1.439008 + (0.000795 * CPM)  where CPM = Counts per Minute
• This formula was obtained from 50 adults (25 males, 25 females) during treadmill exercise at three different speeds (4.8, 6.4, and 9.7 km/hr)
• Reference link
• Special notes: Note that data was collected from adults walking on a treadmill.  According to Freedson et., al., "These data suggest that 1258 counts corresponds to a 1 MET change between 3 and 9 METs using 1951 counts as the 'baseline' to define the 3 MET level" which implies this formula may not produce valid results below 3 METs or above 9 METs.  For the sake of usability in ActiLife, the forumula will provide data when the CPM>=100.  However, no guarantees can be made about this extrapolation.  Contact the author for further information.

ActiLife 5 and 6 can communicate with the following devices:

• GT1M
• GT2M
• GT3M
• GT3X+
• ActiTrainer (research model)
• ActiSleep
• ActiSleep+

ActiGraph wGT3X-BT and wActiSleep-BT devices contain a metallic plate on the back of the battery near the cover which can be used to detect monitor removal when worn against the skin.  At this time, this technique works best for wrist-worn devices.

There is no need to activate the wear sensor.  Data is gathered continuously during normal usage after initialization.  The wear sensor data can be consumed alongside all of ActiLife's built-in wear time validation algorithms using the new wear sense preview graphs.  The wear sensor can detect short device removals (e.g., 10 minute long shower) whereas the built-in algorithms struggle with detecting non-wear periods shorter than an hour in length.

ActiGraph sells special wrist straps for the wGT3X-BT and wActiSleep-BT devices which ensure good contact with the skin for wrist-worn studies. Visit http://www.actigraphcorp.com/product-category/accessories/ for more details.